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HA-tag polyclonal antibody

 
ADI-MSA-106-E 100 µl 313.00 USD
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Product Details

Host:Rabbit
 
Immunogen:HA-tag (YPYDVPDYA) conjugated to KLH.
 
Specificity:Recognizes the HA-tag of HA-tagged recombinant proteins.
 
Applications:ICC, IP, WB
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Epitope tagging is a widely accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope. The Ha-Tag (YPYDVPDYA) corresponds to the partial peptide sequence of the influenza hemagglutinin protein.
 
Regulatory Status:RUO - Research Use Only
 
HA-tag polyclonal antibody Western blot
Western blot analysis of HA-tagged I-kB protein expressed in 293T cells, probed with HAtag pAb
HA-tag polyclonal antibody Immunoprecipitation
Immunoprecipitation analysis of HA tagged I -kB protein from transfectant with HA-tag pAb ; immunocomplex was resolved on SDS-PAGE after immunoprecipitation with the antibody, and immunoblotted with HA -tag pAb.
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HA-tag polyclonal antibody Western blot HA-tag polyclonal antibody Immunoprecipitation

Product Literature References

Ubiquitination of stalled ribosomes enables mRNA decay via HBS-1 and NONU-1 in vivo: P.C. Monem, et al.; PLoS Genet. 19, e101057 (2023), Abstract;

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