Product Details
Host: | Rabbit |
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Immunogen: | HA-tag (YPYDVPDYA) conjugated to KLH. |
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Specificity: | Recognizes the HA-tag of HA-tagged recombinant proteins. |
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Applications: | ICC, IP, WB
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Epitope tagging is a widely accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope. The Ha-Tag (YPYDVPDYA) corresponds to the partial peptide sequence of the influenza hemagglutinin protein. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of HA-tagged I-kB protein expressed in 293T cells, probed with HAtag pAb
Immunoprecipitation analysis of HA tagged I -kB protein from transfectant with HA-tag pAb ; immunocomplex was resolved on SDS-PAGE after immunoprecipitation with the antibody, and immunoblotted with HA -tag pAb.
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Product Literature References
Ubiquitination of stalled ribosomes enables mRNA decay via HBS-1 and NONU-1 in vivo: P.C. Monem, et al.; PLoS Genet.
19, e101057 (2023),
Abstract;
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