Recommended Dilutions/Conditions: | Western Blot (20µg/lane) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Source: | Pooled rat liver samples |
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Formulation: | Liquid. In SDS-PAGE sample buffer, pH 6.8, containing 40mM TRIS-HCl, 1% SDS, 50mM DTT, 7.5% glycerol, and 0.003% bromophenol blue. |
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Applications: | WB
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Application Notes: | Western blot control. |
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Shipping: | Shipped on Dry Ice |
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Long Term Storage: | -80°C |
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Handling: | Avoid freeze/thaw cycles. |
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Scientific Background: | Liver microsomes are subcellular particles derived from the endoplasmic reticulum of hepatic cells. Microsomes are a rich source of heme-containing metabolic enzymes, including heme oxygenases and cytochrome P450 (CYP) enzymes, and are commonly used in the study of xenobiotic drug metabolism. This microsome preparation consists of pooled microsomes isolated from female IGS Sprague-Dawley rat livers. Production Method: The tissue extract was adjusted to 2mg/mL in SDS PAGE sample buffer (40 mM Tris-HCl pH 6.8, 1% SDS, 50 mM DTT, 7.5% glycerol, 0.003% bromophenol blue) and heated for 10 min at 70°C. |
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Regulatory Status: | RUO - Research Use Only |
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