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A431, (cell lysate)

ADI-LYC-A4100-F 200 µg 127.00 USD
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Product Details

Recommended Dilutions/Conditions:Western Blot (20µg/lane)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. In SDS-PAGE sample buffer, pH 6.8, heated for 10 min. at 70°C and containing 40mM TRIS-HCl, 1% SDS, 50mM DTT, 7.5% glycerol, and 0.003% bromophenol blue.
Application Notes:Western blot control.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Handling:Avoid freeze/thaw cycles.
Scientific Background:A431 cells were maintained in Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; fetal bovine serum, 10%. A431. Cells collected from media by centrifuging at 1200 rpm at 4°C in a Beckman GS-6R centrifuge for 7 minutes. Cells were washed twice in PBS and were lysed in MPER Mammalian Protein Extraction Reagent (Pierce catalog# 78501) with a protease inhibitor cocktail. The cell lysate was adjusted to 2mg/mL in SDS PAGE sample buffer (40 mM Tris-HCl pH 6.8, 1% SDS, 50 mM DTT, 7.5% glycerol, 0.003% bromophenol blue) and heated for 10 min at 70°C.
Regulatory Status:RUO - Research Use Only

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