Product Details
| Alternative Name: | Eukaryotic translation initiation factor 2 subunit α |
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| MW: | ~36kDa |
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| Source: | Produced in E. coli. Human eIF2α is fused at the N-terminus to a His-tag. |
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| UniProt ID: | P05198 |
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| Formulation: | Liquid. In 50mM TRIS, pH 7.5, containing 10% glycerol, 0.5% Triton X-100, 300mM potassium chloride, and 4mM DTT. |
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| Purity: | ≥85% (SDS-PAGE; Western blot) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Applications: | WB
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| Application Notes: | Western blot control. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | Eukaryotic translation initiation factor 2α (eIF2α) promotes the binding of initiator tRNA to the 40S ribosomal subunits, and controls translational rates via phosphorylation/dephosphorylation mechanisms. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF2α. Phosphorylated eIF2α inhibits cell growth and induces apoptosis. Phosphorylation of eIF2α occurs under a variety of conditions, including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses. eIF2 exists as a heterotrimeric protein composed of 3 nonidentical subunits: α (36kDa), β (38kDa), and γ (52kDa). |
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| Regulatory Status: | RUO - Research Use Only |
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Western Blot Analysis of eIF2α (human), (recombinant) (His-tag) probed with eIF2α polyclonal antibody: Lane 1: MW Marker, Lane 2: 200ng, Lane 3: 400ng.
SDS-PAGE Analysis of eIF2α (human), (recombinant) (His-tag): Lane 1: MW Marker, Lane 2: 0.5μg, Lane 3: 1.0μg, Lane 4: 2.0μg, Lane 5: 3.0μg.
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Product Literature References
Chronic Hyperinsulinemia increases myoblast proliferation in fetal sheep skeletal muscle: L.D. Brown, et al.; Endocrinology
157, 2447 (2016),
Application(s): Western blot,
Abstract;
Full Text
General Literature References
Double-stranded RNA-activated protein kinase (PKR) is negatively regulated by 60S ribosomal subunit protein L18: K.U. Kumar, et al.; Mol. Cell. Biol.
19, 1116 (1999),
Abstract;
Effect of brain ischemia and reperfusion on the localization of phosphorylated eukaryotic initiation factor 2 alpha: D.J. DeGracia, et al.; J. Cereb. Blood Flow Metab.
17, 1291 (1997),
Abstract;
Localization of eukaryotic initiation factor 2 in neuron primary cultures and established cell lines: M.V. Lobo, et al.; Histochem. J.
29, 453 (1997),
Abstract;
