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iNOS polyclonal antibody

ADI-KAS-NO001-D 50 µg 243.00 USD
ADI-KAS-NO001-F 200 µg 403.00 USD
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Product Details

Alternative Name:NOS2, NOS II, Nitric oxide synthase (inducible), Hepatocyte NOS
Immunogen:Synthetic peptide corresponding to aa 1131-1144 of mouse iNOS.
UniProt ID:P29477
Gene/Protein Identifier:NM_010927 (RefSeq)
Source:Purified from rabbit serum.
Species reactivity:Mouse, Rat
Applications:IP, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~130kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.
Regulatory Status:RUO - Research Use Only
iNOS polyclonal antibody Western blot
Western blot analysis: rat brain extract, probed with iNOS, pAb.
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iNOS polyclonal antibody Western blot

Product Literature References

C57BL/6N Mice Are More Resistant to Ehrlich Ascites Tumors Than C57BL/6J Mice: The Role of Macrophage Nitric Oxide: S. Kalish, et al.; Med. Sci. Monit. Basic Res. 21, 235 (2015), Application(s): Western Blot, Abstract; Full Text
Dietary Supplementation of Genistein Attenuates Inflammatory Responses and Oxidative Stress during Cutaneous Wound Healing in Diabetic Mice: H. Eo, et al.; J. Agr. Sci. 7, 80 (2015), Application(s): Western Blotting, Full Text
Phenolic content of extra virgin olive oil is essential to restore endothelial dysfunction but not to prevent vascular inflammation in atherosclerotic lesions of Apo E deficient mice: C. Claro, et al.; J. Funct. Foods 15, 126 (2015), Application(s): Western Blot,
Protective effect of a novel Rho kinase inhibitor WAR-5 in experimental autoimmune encephalomyelitis by modulating inflammatory response and neurotrophic factors: Y.H. Li, et al.; Exp. Mol. Pathol. 99, 220 (2015), Application(s): Injection into mouse abdominal cavity, Abstract;
Proapoptotic nitric oxide production in amyloid β protein-treated cerebral microvascular endothelial cells: M. Oike, et al. ; Microcirculation 14, 89 (2007), Application(s): WB using bovine cell lysates, Abstract;

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