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Methylated Lysine polyclonal antibody (HRP conjugate)

ADI-KAP-TF1213-E 100 µg 598.00 USD
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Product Details

Immunogen:Methylated KLH.
Species reactivity:Species independent
Applications:ELISA, IHC, WB
Recommended Dilutions/Conditions:ELISA (0.1µg/ml)
Western Blot (0.2-0.5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Immunoaffinity purified.
Formulation:Liquid. In HRP stabilizing buffer with 50% glycerol.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Acetylation and methylation of lysine are important post-translational modifications that regulate numerous protein-protein and protein-DNA interactions. Lysine acetylation and methylation involves the transfer of acetylCoA, or one or more methyl groups, to the e-amino group of lysine by modifying enzymes and cofactors. Histones and transcription factors are the primary targets of lysine acetylation and methylation, with either modification capable of inducing gene silencing or expression due to differential regulation of cofactors. For example, varying degrees of mono-, di-, and tri-methylation or acetylation of histone H3 at lysine residue 9 are known to demark distinct chromatin regions during various states of gene activation (methylation) or repression (acetylation).
Regulatory Status:RUO - Research Use Only
Methylated Lysine polyclonal antibody (HRP conjugate) Western blot
Western blot analysis of the methylated protein pattern in whole HeLa cell with Methylated Lysine pAb, HRP conjugate. Left: Signal of methylated proteins; Middle: Signal of the methylated proteins was competitively suppressed by 50µg/ml of methylated BSA; Right: Signal of the methylated proteins was competitively suppressed by 10µg/ml of methylated BSA.
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Methylated Lysine polyclonal antibody (HRP conjugate) Western blot

Product Literature References

Smyd1b is required for skeletal and cardiac muscle function in zebrafish: H. Li, et al.; Mol. Biol. Cell. 24, 3511 (2013), Application(s): Western blot, Abstract; Full Text

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