Product Details
Alternative Name: | AcK |
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Host: | Rabbit |
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Immunogen: | Acetylated KLH. |
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Species reactivity: | Species independent
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Applications: | ELISA, IF, IP, WB
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Recommended Dilutions/Conditions: | ELISA (1:1,000) Immunofluorescence (1:50) Immunoprecipitation (1:25) Western Blot (1:250) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS, 50% glycerol. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Acetylation and methylation of lysine are important post-translational modifications that regulate numerous protein-protein and protein-DNA interactions. Lysine acetylation and methylation involves the transfer of acetylCoA, or one or more methyl groups, to the e-amino group of lysine by modifying enzymes and cofactors. Histones and transcription factors are the primary targets of lysine acetylation and methylation, with either modification capable of inducing gene silencing or expression due to differential regulation of cofactors. For example, varying degrees of mono-, di-, and tri-methylation or acetylation of histone H3 at lysine residue 9 are known to demark distinct chromatin regions during various states of gene activation (methylation) or repression (acetylation). |
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Regulatory Status: | RUO - Research Use Only |
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Immunofluorescent analysis of TSA treated (right - 100ng/ml, 24 hours) and non-treated (left) human MMRU melanoma cells using Acetylated Lysine (RS2) pAb.
Immunoprecipitation analysis of acetylated BSA with Acetylated Lysine (RS1) pAb in ng.
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Product Literature References
Histone deacetylase inhibitors synergize p300 autoacetylation that regulates its transactivation activity and complex formation: N. Sang, et al. ; Cancer Res.
67, 2256 (2007),
Application(s): NA using human cell lysates,
Abstract;
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