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IKK β polyclonal antibody

ADI-KAP-TF118-E 100 µg 340.00 USD
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Product Specification

Alternative Name:IκB kinase
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human IKKβ. The sequence differs from mouse IKKβ by one amino acid.
UniProt ID:O14920
GenBank ID:AF029684.1
Species reactivity:Human
Applications:ICC, WB
Recommended Dilutions/Conditions:Western Blot (2µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~87kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:Nuclear factor κ B (NF-κB) is a ubiquitous and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-κB activity. IkappaB is phosphorylated by IkappaB kinase (IKK) complex that contains IKKalpha, IKKbeta, and IKKgamma. A molecule in the IKK complex was identified and designated IKKepsilon/IKK-iota.
Regulatory Status:RUO - Research Use Only
IKK β polyclonal antibody Immunocytochemistry
Immunocytochemistry analysis of HeLa cells using IKK beta antibody at 10µg/ml.
IKK β polyclonal antibody Western blot
Western blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.
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IKK β polyclonal antibody Immunocytochemistry IKK β polyclonal antibody Western blot

Product Literature References

The NEDD8-activating enzyme inhibitor MLN4924 sensitizes a TNFR1+ subgroup of multiple myeloma cells for TNF-induced cell death: M. El-Mesery, et al.; Cell Death Dis. 10, 611 (2019), Abstract; Full Text

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