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NFκB p50 polyclonal antibody

ADI-KAP-TF112-E 100 µg 304.00 USD
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Product Specification

Alternative Name:NFKB1
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of mouse NF-κB.
UniProt ID:P25799
GenBank ID:M57999
Source:Purified from rabbit serum.
Species reactivity:Mouse, Rat
Recommended Dilutions/Conditions:Western Blot (0.5µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects bands of ~50kDa and ~105kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In borate buffered saline, pH 8.2.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Nuclear factor κB (NF-κB) was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of the kappa light chain gene in B lymphocytes. NF-κB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkappaB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines, and viral infection, IkappaB is phosphorylated and degraded by the proteosome. The active NF-κB heterodimer is translocated into the nucleus and induces gene expression.
Regulatory Status:RUO - Research Use Only
NFκB p50 polyclonal antibody Western blot
Western blot analysis of rat spleen tissue extract, probed with NF-kappaB (p50) pAb.
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NFκB p50 polyclonal antibody Western blot

Product Literature References

Members of the Toll-Like Receptor Family of Innate Immunity Pattern-Recognition Receptors Are Abundant in the Male Rat Reproductive Tract: M.A. Palladino, et al.; Biol. Reprod. 76, 958 (2007), Abstract; Full Text

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