Product Details
Host: | Rabbit |
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Immunogen: | Phosphothreonine conjugated to KLH. |
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Species reactivity: | Species independent
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Applications: | ELISA, IP, WB
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Recommended Dilutions/Conditions: | ELISA (0.5µg/ml) Immunoprecipitation (10µg/mg protein) Western Blot (2µg/ml, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Immunoaffinity purified. |
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Formulation: | Liquid. In PBS, pH 7.0 containing 50% glycerol. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Protein phosphorylation is a complex biochemical process that is regulated by protein kinases that catalyze the transfer of the gamma-P of ATP to a recipient protein that acts as a substrate. This post-translational modification is primarily directed onto serine, threonine, and tyrosine amino acid residues within a protein. Changes in the phosphorylation status of phosphoproteins can alter their biological function, and aberrations in signal transduction cascades have been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of mouse spleen extract probed with Prod. No. ADI-KAP-ST211. Left Lane: Vanadium treated; Right lane: Untreated.
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Product Literature References
The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x: C. Sette, et al. ; J. Cell Biol.
176, 929 (2007),
Application(s): WB using human cell lysates,
Abstract;
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