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Phosphothreonine polyclonal antibody

ADI-KAP-ST211-E 100 µg 387.00 USD
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Product Details

Immunogen:Phosphothreonine conjugated to KLH.
Species reactivity:Species independent
Applications:ELISA, IP, WB
Recommended Dilutions/Conditions:ELISA (0.5µg/ml)
Immunoprecipitation (10µg/mg protein)
Western Blot (2µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Immunoaffinity purified.
Formulation:Liquid. In PBS, pH 7.0 containing 50% glycerol.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Protein phosphorylation is a complex biochemical process that is regulated by protein kinases that catalyze the transfer of the gamma-P of ATP to a recipient protein that acts as a substrate. This post-translational modification is primarily directed onto serine, threonine, and tyrosine amino acid residues within a protein. Changes in the phosphorylation status of phosphoproteins can alter their biological function, and aberrations in signal transduction cascades have been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.
Regulatory Status:RUO - Research Use Only
Phosphothreonine polyclonal antibody Western blot
Western blot analysis of mouse spleen extract probed with Prod. No. ADI-KAP-ST211. Left Lane: Vanadium treated; Right lane: Untreated.
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Phosphothreonine polyclonal antibody Western blot

Product Literature References

The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x: C. Sette, et al. ; J. Cell Biol. 176, 929 (2007), Application(s): WB using human cell lysates, Abstract;

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