Product Specification
Alternative Name: | Protein kinase G, cGMP-dependent protein kinase 1 |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to a portion of human PKG1β, with the subsitiution of a tryptophan residue for tyrosine at residue 9. |
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UniProt ID: | Q13976-2 |
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GenBank ID: | Y07512 |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human, Mouse, Rat Bovine
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Applications: | ICC, IHC, IP, WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~80kDa by Western blot. |
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Purity Detail: | Peptide affinity purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Cyclic GMP-dependent protein kinases (PKGs or cGKs) are classified into two types, PKGI and PKGII. Studies have shown that PKGs are highly homologous to PKAs; phosphorylation of cellular proteins by both families of kinases leads to alterations in calcium mobilization, protein phosphatase activity, ion channel function, gene transcription, smooth muscle contractility, and platelet aggregation. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Prod. No. ADI-KAP-PK002: Lane 1: MW marker, Lane 2: HeLa cell lysate, Lane 3: Rat brain tissue extract, Lane 4: MDBK cell lysate.
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Product Literature References
The Golgi apparatus regulates cGMP-dependent protein kinase I compartmentation and proteolysis: S. Kato, et al.; Am. J. Physiol. Cell Physiol.
308, C944 (2015),
Abstract;
Full Text
Alternative splicing of cGMP-dependent protein kinase I in angiotensin-hypertension: novel mechanism for nitrate tolerance in vascular smooth muscle: J.M. Simard, et al. ; Circ. Res.
93, 805 (2003),
Application(s): IHC, WB using rat samples,
Abstract;
Protein kinase G activation of K(ATP) channels in human-cultured prostatic stromal cells: J.M. Haynes, et al. ; Cell. Signal.
14, 1023 (2002),
Application(s): ICC using human samples,
Abstract;
Exisulind induction of apoptosis involves guanosine 3',5'-cyclic monophosphate phosphodiesterase inhibition, protein kinase G activation, and attenuated beta-catenin: R. Pamukcu, et al. ; Cancer Res.
60, 3338 (2000),
Application(s): WB using human samples,
Abstract;
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