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eNOS polyclonal antibody

ADI-KAP-NO020-F 200 µg 457.00 USD
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Product Details

Alternative Name:NOS3, cNOS, NOS III, Nitric oxide synthase (endothelial)
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human eNOS.
UniProt ID:P29474
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~135kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.
Regulatory Status:RUO - Research Use Only
eNOS polyclonal antibody Immunohistochemistry
IHC Analysis: Human placenta tissue stained with eNOS, pAb at 10µg/ml.
eNOS polyclonal antibody Western blot
Western blot analysis: Lane 1 MW marker, Lane 2: Rat Brain, and Lane 3: Mouse Brain probed with eNOS, pAb.
eNOS polyclonal antibody Immunohistochemistry
IHC Analysis: Human spleen tissue stained with eNOS, pAb at 10µg/ml.
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eNOS polyclonal antibody Immunohistochemistry eNOS polyclonal antibody Western blot eNOS polyclonal antibody Immunohistochemistry

Product Literature References

Enteric neuroanatomy and smooth muscle activity in the western diamondback rattlesnake (Crotalus atrox): T. Kohl, et al.; Front. Zool. 20, s12983 (2023), Abstract;
Blockade on Lin28a Prevents Cognitive Impairment and Disruption of the Blood-Brain Barrier Induced by Chronic Cerebral Hypoperfusion: J.M. Lee, et al.; Biomedicines 10, 852 (2022), Abstract;
Fermented garlic extract decreases blood pressure through nitrite and sGC-cGMP-PKG pathway in spontaneously hypertensive rats: B.M. Park, et al.; J. Funct. Foods 22, 156 (2016), Application(s): Western blot,
Sestrin 2 and AMPK connect hyperglycemia to Nox4-dependent endothelial nitric oxide synthase uncoupling and matrix protein expression: A. Eid, et al.; Mol. Cell. Biol. 33, 3439 (2013), Application(s): WB of rat glomerular mesangial cells, Abstract; Full Text

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