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MKK6 polyclonal antibody

ADI-KAP-MA014-C 25 µg 120.00 USD
ADI-KAP-MA014-E 100 µg 295.00 USD
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Product Specification

Alternative Name:MAP2K6, MAP kinase kinase 6
Immunogen:Synthetic peptide corresponding to a portion of human MEK6.
UniProt ID:P52564
GenBank ID:U39656
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Applications:IHC, IP, WB
Recommended Dilutions/Conditions:Immunohistochemistry (5µg/ml)
Immunoprecipitation (4µg/ml)
Western Blot (4µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~38kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In borate buffered saline, pH 8.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Members of the MAP kinase family of protein kinases (e.g. p38 and Jnk) are subject to dual phosphorylation of a conserved T-X-Y motif in their activation domain by upstream members of the MAP/ERK kinase (MEK/MKK) family. MKK3 and MKK6 phosphorylate p38 in response to cytokine stimulation and cellular stress, while MKK7 and MKK4 activate the Jnk kinases by phosphorylation of the threonine and tyrosine residues, respectively.
Regulatory Status:RUO - Research Use Only
MKK6 polyclonal antibody Western blot
Western blot analysis of mouse brain tissue extract, probed with MKK6 pAb.
MKK6 polyclonal antibody Immunohistochemistry
Immunohistochemistry analysis of MKK6. There is prominent MKK6 in association with neurons containing neurofibrillary pathology in hippocampal sections (CA1 area) from an AD patient with granular structure heavily stained (right). MKK6 is barely detected in control brain (left).
MKK6 polyclonal antibody Immunohistochemistry
Immunohistochemistry analysis of human breast cancer tissue with MKK6 pAb.
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MKK6 polyclonal antibody Western blot MKK6 polyclonal antibody Immunohistochemistry MKK6 polyclonal antibody Immunohistochemistry

Product Literature References

Proteasome Activation by Small Molecules: Y. Leestemaker, et al.; Cell Chem. Biol. 24, 725 (2017), Abstract;

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