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MEK1 polyclonal antibody

ADI-KAP-MA010-E 100 µg 372.00 USD
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Product Details

Alternative Name:MKK1, MAP kinase kinase 1
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of human MEK1. The sequence is completely conserved in mouse, rat, hamster, rabbit and Xenopus.
UniProt ID:Q02750
GenBank ID:L05624
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Applications:IF, IHC, WB
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Western Blot (1µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~48kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:MEK is a dual specificity kinase capable of phosphorylating both tyrosine and threonine residues. The MEK family, which is also known as MAP kinase kinase, phosphorylates MAP kinases on the conserved T-X-Y motif; phosphorylation of MAPK by MEK results in an increase in MAPK activity. MEK is involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis.
Regulatory Status:RUO - Research Use Only
MEK1 polyclonal antibody Western blot
Western blot analysis of MEK1, pAb (Prod. No. ADI-KAP-MA010): Lane 1: MW marker; Lane 2: HeLa (heat shocked) (Prod. No. ADI-LYT-HL-101); Lane 3: Rat spleen extract; Lane 4: Mouse brain extract (Prod. No. ADI-LYT-MB100)
MEK1 polyclonal antibody Immunohistochemistry
Immunohistochemistry analysis of human breast cancer tissue with MEK1 pAb.
MEK1 polyclonal antibody Immunofluorescence
Immunofluorescent analysis (confocal) staining of HeLa cells using MEK1 pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.
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MEK1 polyclonal antibody Western blot MEK1 polyclonal antibody Immunohistochemistry MEK1 polyclonal antibody Immunofluorescence

Product Literature References

Role of ether-linked lysophosphatidic acids in ovarian cancer cells: Y. Xu, et al. ; J. Lipid Res. 43, 463 (2002), Application(s): WB using human samples, Abstract;

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