Product Details
Alternative Name: | MKK5, MAP kinase kinase 5 |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to a portion of human MEK5. The sequence is completely conserved in rat. |
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UniProt ID: | Q13163 |
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GenBank ID: | U71088 |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human, Mouse, Rat Dog, Monkey, Rabbit, Sheep, Xenopus
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Applications: | IHC, WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~42kDa by Western blot. |
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Purity Detail: | Peptide affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | MEK is a dual specificity kinase capable of phosphorylating both tyrosine and threonine residues. The MEK family, which is also known as MAP kinase kinase, phosphorylates MAP kinases on the conserved T-X-Y motif; phosphorylation of MAPK by MEK results in an increase in MAPK activity. MEK is involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis. |
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Regulatory Status: | RUO - Research Use Only |
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Western Blot analysis of MEK5, pAb (Prod. No. ADI-KAP-MA003): Lane 1: Jurkat, Lane 2: Mouse Testes, Lane 3: Rat Brain
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Product Literature References
Clinical, genetic and pharmacological data support targeting the MEK5/ERK5 module in lung cancer: A. Sánchez-Fdez, et al.; NPJ Precis. Oncol.
5, 78 (2021),
Abstract;
MEK5 overexpression is associated with metastatic prostate cancer, and stimulates proliferation, MMP-9 expression and invasion: H.Y. Leung, et al. ; Oncogene
22, 1381 (2003),
Application(s): IHC using human samples,
Abstract;
MEKK3 directly regulates MEK5 activity as part of the big mitogen-activated protein kinase 1 (BMK1) signaling pathway: J.D. Lee, et al. ; J. Biol. Chem.
274, 36035 (1999),
Application(s): WB using mouse samples,
Abstract;
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