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Erk1/2 polyclonal antibody

ADI-KAP-MA001-D 50 µg 216.00 USD
ADI-KAP-MA001-F 200 µg 510.00 USD
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Product Details

Alternative Name:MAPK2/3, MAP kinase 2/3
Immunogen:Synthetic peptide corresponding to a portion of rat Erk1. The sequence is completely conserved in hamster.
UniProt ID:P21708
GenBank ID:M61177
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Drosophila, Sheep, Xenopus
Applications:Flow Cytometry, IHC (PS), IP, WB
Recommended Dilutions/Conditions:Flow Cytometry (10µg/ml)
Immunohistochemistry (paraffin sections, 1:50)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a bands of ~42kDa (Erk2) and ~44kDa (Erk1) by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:The Extracellular regulated kinases, Erk-1 and Erk-2, both play a critical role in cellular proliferation. These extracellular kinases, are known to reside downstream of the Mitogen activated protein kinase/Extracellular protein kinases referred to as MEK. Both MEK-1 and MEK-2 phosphorylate and activate Erk on the conserved TEY motif within the activation loop of the kinase.
Regulatory Status:RUO - Research Use Only
Erk1/2 polyclonal antibody Immunohistochemistry
Immunohistochemistry analysis: Human placenta tissue stained with Erk1/2, pAb at 10µg/ml.
Erk1/2 polyclonal antibody Flow Cytometry
Flow cytometry analysis of U251 cells using Erk1/2 pAb (right) and control rabbit IgG (left).
Erk1/2 polyclonal antibody Western blot
Western blot analysis of human A431 fibroblast cell lysate, probed with Erk1/2 pAb.
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Erk1/2 polyclonal antibody Immunohistochemistry Erk1/2 polyclonal antibody Flow Cytometry Erk1/2 polyclonal antibody Western blot

Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
EGF-mediated EGFR/ERK signaling pathway promotes germinative cell proliferation in Echinococcus multilocularis that contributes to larval growth and development: Z. Cheng, et al.; PLoS Negl. Trop. Dis. 11, e0005418 (2017), Abstract; Full Text
Cognitive impairments following cranial irradiation can be mitigated by treatment with a tropomyosin receptor kinase B agonist: P. Yang, et al.; Exp. Neurol. 279, 178 (2016), Application(s): Immunoblotting, Abstract;
Dendritic Cell Differentiation Induced by a Self-Peptide Derived from Apolipoprotein E: B. Singh, et al. ; J. Immunol. 181, 6859 (2008), Application(s): WB using mouse cell lysates, Abstract;
Reduction of chronic allograft nephropathy by inhibition of extracellular signal-regulated kinase 1 and 2 signaling: C. Du, et al. ; Am. J. Physiol. Renal Physiol. 295, F672 (2008), Application(s): WB, IHC using mouse tissue, Abstract;
Surface cytotoxic T lymphocyte-associated antigen 4 partitions within lipid rafts and relocates to the immunological synapse under conditions of inhibition of T cell activation: J. Madrenas, et al. ; J. Exp. Med. 195, 1337 (2002), Application(s): WB using human samples, Abstract;
Stress-induced inhibition of ERK1 and ERK2 by direct interaction with p38 MAP kinase: S. Pelech, et al. ; J. Biol. Chem. 276, 6905 (2001), Application(s): IP using human samples, Abstract;
Zap-70-independent Ca(2+) mobilization and Erk activation in Jurkat T cells in response to T-cell antigen receptor ligation: R.L. Wange, et al. ; Mol. Cell. Biol. 21, 7137 (2001), Application(s): WB using human samples, Abstract;

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