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RAB5 polyclonal antibody

ADI-KAP-GP006-E 100 µg 328.00 USD
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Product Details

Alternative Name:Ras-related protein Rab-5
Immunogen:Synthetic peptide corresponding to a portion of human Rab5. The sequence is completely conserved in dog.
UniProt ID:P20339 (human), P18066 (dog)
GenBank ID:M35520 (dog)
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Guinea pig, Hamster, Monkey
Applications:ICC, IHC, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~26kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:The Rab family of GTP-binding proteins are members of the Ras superfamily of small G proteins, and participate in multiple steps of the membrane trafficking. Rab3A participates in the fusion of synaptic vesicles during neurotransmitter release, a process regulated by GDP/GDP exchange cofactors such as GAP, GDI, GRF, and rabphilin. Rab4 regulates recycling of proteins from the early endosome to the surface of many cell types, regulating glucose transporter and transferrin receptor cell surface localization. Rab5 regulates the fusion of plasma membrane-derived clathrin-coated vesicles with early endosomes and homotypic fusion among early endosomes.
Regulatory Status:RUO - Research Use Only
RAB5 polyclonal antibody Western blot
Western blot analysis of human HeLa (heat shocked) cell lysate, probed with Rab5 pAb.
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RAB5 polyclonal antibody Western blot

Product Literature References

Accumulation of cholera toxin and GM1 ganglioside in the early endosome of Niemann-Pick C1-deficient cells: K. Ohno, et al. ; PNAS 98, 12391 (2001), Application(s): ICC using human samples, Abstract;
Intra-endosomal pH-sensitive recruitment of the Arf-nucleotide exchange factor ARNO and Arf6 from cytoplasm to proximal tubule endosomes: V. Marshansky, et al. ; J. Biol. Chem. 276, 18540 (2001), Application(s): IHC , Abstract;

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