eIF2 α polyclonal antibody

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ADI-KAP-CP130-D	50 µg	275.00 USD

ADI-KAP-CP130-F	200 µg	540.00 USD
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Alternative Name:	Eukaryotic translation initiation factor 2 subunit α

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to a portion of rat eIF2-α.

UniProt ID:	P68101

Source:	Purified from rabbit serum.

Species reactivity:	Human, Mouse, Rat Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep

Applications:	ICC, IHC (PS), WB

Recommended Dilutions/Conditions:	Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~36kDa by Western blot.

Purity Detail:	Immunoaffinity purified.

Formulation:	Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Scientific Background:	EIF-2α, also known as eukaryotic translation initiation factor 2α, promotes the binding of the initiator tRNA to the 40S ribosomal subunits and controls translational rates via phosphorylation/dephosphorylation mechanisms. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF-2α. Phosphorylated eIF-2α inhibits cell growth and induces apoptosis. Phosphorylation of eIF-2α occurs under a variety of conditions including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses. EIF-2 exists as a heterotrimeric protein composed of 3 nonidentical subunits, alpha (36 kDa), beta (38 kDa), and gamma (52 kDa).

Regulatory Status:	RUO - Research Use Only

eIF2 α polyclonal antibody Immunohistochemistry

IHC Analysis: Human prostate tissue stained with eIF2 α, pAb at 10µg/ml.

IHC Analysis: Human pancreas tissue stained with eIF2α, pAb at 10µg/ml.

IHC Analysis: Human vessel tissue stained with eIF2 α, pAb at 10µg/ml.

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PERK prevents hepatic lipotoxicity by activating the p62-ULK1 axis-mediated noncanonical KEAP1-Nrf2 pathway: D.H. Lee, et al.; Redox Biol. 1016, 102235 (2022), Abstract;

Musashi-1 maintains blood-testis barrier structure during spermatogenesis and regulates stress granule formation upon heat stress: S. ErLin, et al.; Mol. Biol. Cell 26, 1947 (2015), Application(s): Western Blotting, Abstract; Full Text

Prolonged maternal amino acid infusion in late-gestation pregnant sheep increases fetal amino acid oxidation: L. Brown, et al. ; Am. J. Physiol. Endocrinol. Metab. 297, E638 (2009), Application(s): WB using sheep tissue, Abstract;

Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules: P. Anderson, et al. ; Mol. Biol. Cell 13, 195 (2002), Application(s): ICC, WB using human samples, Abstract;

PERK mediates cell-cycle exit during the mammalian unfolded protein response: J.A. Diehl, et al. ; PNAS 97, 12625 (2000), Application(s): WB using mouse samples, Abstract;

Datasheet
SDS
Certificate of Analysis

By target:

eIF

By biological activity:

eIF Polyclonal antibody

By catalog section:

Protein Synthesis

HeLa, (cell lysate)

Human, WB | Print as PDF

ADI-LYC-HL100-F

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