Host: | Rabbit |
|
Immunogen: | Synthetic peptide corresponding to a portion of human Aurora B phosphorylated at Thr232. |
|
UniProt ID: | Q96GD4 |
|
GenBank ID: | AAH80581 |
|
Source: | Purified from rabbit serum. |
|
Species reactivity: | Human
|
|
Crossreactivity: | Weakly cross-reacts with mouse, rat, bovine and pig Aurora B. |
|
Applications: | IHC (PS), WB
|
|
Recommended Dilutions/Conditions: | Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Purity Detail: | Peptide affinity purified. |
|
Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
|
Handling: | Avoid freeze/thaw cycles. |
|
Shipping: | Shipped on Blue Ice |
|
Long Term Storage: | -20°C |
|
Scientific Background: | Aurora A, B, and C have emerged as key mitotic regulators of mammalian centrosome function, chromosome segregation and cytokinesis. Aurora B is one of several chromosomal passenger proteins involved in chromosome alignment at the metaphase plate and cytokinesis. Phosphorylation of a threonine residue in the Aurora activation loop (Thr232 in human Aurora B) is believed to be an essential regulatory mechanism in Aurora kinase activation. |
|
Regulatory Status: | RUO - Research Use Only |
|