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TIF1 β monoclonal antibody (20A1)

ADI-KAM-TF200-D 50 µg 185.00 USD
ADI-KAM-TF200-F 200 µg 340.00 USD
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Product Specification

Alternative Name:Transcription intermediary factor 1β, E3 SUMO-protein ligase TRIM28
Immunogen:Recombinant human TIF1β (N-terminal half).
UniProt ID:Q13263
Source:Purified from hybridoma tissue culture supernatant.
Species reactivity:Human
Recommended Dilutions/Conditions:Western Blot (1:3,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~110kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Transcriptional intermediary factors (TIFs) are a group of transcriptional coactivators and corepressors that regulate gene expression by modulating chromatin structure and assembly of transcription initiation complexes. TIF1 beta (also known as KAP-1 or KRIP-1) is a member of the TIF1 subfamily of chromatin-associated TIFs that play a key role in many developmental and physiological processes. The three mammalian TIF1 proteins (TIF1 alpha, beta and gamma) share three conserved domains. An N-terminal RING-B box coiled coil (RBCC) domain most likely attributes to intermolecular structure, while two characteristic chromatin-targeting domains, a plextrin homology (PH) finger motif and bromodomain, are found in the C-terminus. TIF1 beta acts as a transcriptional repressor by modulating histone deacetlyation, histone H3 Ly-9 methylation, and recruitment of HP1 proteins. TIF1 beta was shown to form homo-oligomers in vitro, but does not form hetero-oligomers with TIF1 alpha or TIF1 gamma.
Regulatory Status:RUO - Research Use Only
TIF1 β monoclonal antibody (20A1) Western blot
Western blot analysis of TIF1β mAb (20A1): Lane 1: MW marker, Lane 2: HeLa, Lane3: A-431, Lane 4: PC-3.
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TIF1 β monoclonal antibody (20A1) Western blot

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