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β-Catenin monoclonal antibody (12F7)

ADI-KAM-ST001-E 100 µg 287.00 USD
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Product Details

Alternative Name:CTNNB
Immunogen:Recombinant β-catenin (N-terminal fragment).
UniProt ID:P35222 (human), Q02248 (mouse), Q9WU82 (rat)
Species reactivity:Human, Mouse, Rat
Applications:IHC, IP, WB
Recommended Dilutions/Conditions:Immunohistochemistry (1-5µg/ml)
Immunoprecipitation (2µg/200-300µl cell extract)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects bands of ~97kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:β-Catenin, a vertebrate homologue of Drosophila armadillo encoded by the CTNNB1 gene, performs two functions in the cell: mediating cell-cell adhesion by association between E-cadherin and the cytoskeleton, and acting as a downstream transcriptional activator of Wnt signaling, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate.  In the absence of Wnt signaling, glycogen synthase kinase-3β (GSK-3β) phosphorylates any free β-catenin at the amino terminus.  This results in adenomatous polyposis coli tumor suppressor protein (APC)-mediated β-catenin degradation via the ubiquitin-proteosome pathway.  Wnt signaling stabilizes β-catenin by inhibiting the activity of GSK-3β and preventing β-catenin degradation.  β-catenin forms complexes with DNA-binding proteins such as T-cell factor (Tcf) and lymphocyte-enhancer factor (Lef), which promote  expression of a variety of target genes, including c-myc and cyclin D1.
Regulatory Status:RUO - Research Use Only
β-Catenin monoclonal antibody (12F7) Western blot
Western blot analysis of A431 cells (1), Hela cells (2), NIH/3T3 cells (3), C2C12 cells (4) and Rat-1 cells (5) probed with β-catenin mAb (12F7)
β-Catenin monoclonal antibody (12F7) Immunoprecipitation
Immunoprecipitation of Rat-1 cells with normal mouse IgG (1) or β-catenin mAb (12F7 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with β-catenin mAb (12F7).
β-Catenin monoclonal antibody (12F7) Immunohistochemistry
Immunohistochemical detection of gastric epithelium β-catenin on paraffin embedded sections with β-Catenin mAb (12F7).
β-Catenin monoclonal antibody (12F7) Immunohistochemistry
Immunohistochemical detection of colorectal cancer β-catenin on paraffin embedded sections with β-catenin mAb (12F7).
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β-Catenin monoclonal antibody (12F7) Western blot β-Catenin monoclonal antibody (12F7) Immunoprecipitation β-Catenin monoclonal antibody (12F7) Immunohistochemistry β-Catenin monoclonal antibody (12F7) Immunohistochemistry

Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;

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