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Nucleolin monoclonal antibody (4E2)

ADI-KAM-CP100-E 100 µg 414.00 USD
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Product Details

Immunogen:Human nucleolin from Raji cell extract.
UniProt ID:P19338
Species reactivity:Human
Applications:Flow Cytometry, ICC, IHC, WB
Recommended Dilutions/Conditions:Flow Cytometry (5-10µg/ml final concentration)
Immunocytochemistry (1-10µg/ml)
Immunohistochemistry (1-10µg/ml)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~100kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Nucleolin (identical to human DNA helicase IV) is an abundant, non-ribosomal protein of the nucleolus involved in the organization of nuclear chromatin, rDNA transcription, packaging of pre-RNA, ribosome assembly, nucleocytoplasmic transport, cytokinesis, nucleogenesis, and apoptosis. A predominantly nuclear protein, the presence of nucleolin at the surface of cancer cells suggests that it could be valuable as a marker for the diagnosis of cancer.
Regulatory Status:RUO - Research Use Only
Nucleolin monoclonal antibody (4E2) Western blot
Western blot analysis of HL60 (1) and ZR-75-1 (2) cell extracts, probed with Nucleolin mAb (4E2).
Nucleolin monoclonal antibody (4E2) Immunohistochemistry
Immunohistochemistry analysis of nuclear staining of colon cancer sections using Nucleolin mAb (4E2).
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Nucleolin monoclonal antibody (4E2) Western blot Nucleolin monoclonal antibody (4E2) Immunohistochemistry

Product Literature References

Ribosome biogenesis-based predictive biomarkers in endocrine therapy (Anastrozole) combined with mTOR inhibitor (Vistusertib) in endometrial cancer: translational study from the VICTORIA trial in collaboration with the GINECO group: N. Mourks, et al.; Mol. Oncol. 17, 27 (2023), Abstract;
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
Centrosomal nucleolin is required for microtubule network organization: X. Gaume, et al.; Cell Cycle 14, 902 (2015), Abstract; Full Text
Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA: M. Schertzer, et al.; Nucleic Acids Res. 43, 1834 (2015), Abstract; Full Text

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