Product Details
| Clone: | 9F3 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Synthetic peptide corresponding to a portion of human Histone H2AX phosphorylated at Ser139. |
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| UniProt ID: | P16104 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~16kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The core histones form an octamer, which is made up of one H3-H4 tetramer and two H2A-H2B dimers. The N-terminal tail of core histones undergoes different post-transcriptional modifications including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. |
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| Technical Info/Product Notes: | Patents: USPN 6,362,317 and 6,884,873 |
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| Regulatory Status: | RUO - Research Use Only |
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![[pSer139]Histone H2AX monoclonal antibody (9F3) Western blot](https://www.enzolifesciences.com/fileadmin/files/image/ADI-KAM-CC255_WB.jpg "[pSer139]Histone H2AX monoclonal antibody (9F3) Western blot")
Western blot analysis of [pSer139] Histone H2AX, mAb (9F3) (Prod. No. ADI-KAM-CC255): Lane 1: MW marker; Lane 2: Jurkat cell lysate; Lane 3: Jurkat cell lysate treated with staurosporine; Lane 4: 3T3 cell lysate; Lane 5: CHO-K1 cell lysate; Lane 6: Rat-2 cell lysate.
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Product Literature References
Identification of CETP as a molecular target for estrogen positive breast cancer cell death by cholesterol depleting agents: L. Esau, et al.; Genes Cancer
7, 309 (2016),
Abstract;
Full Text
The bipartite Rac1 guanine nucleotide exchange factor engulfment and cell motility 1/dedicator of cytokinesis 180 (Elmo1/Dock180) protects endothelial cells from apoptosis in blood vessel development: K. Schaeker, et al.; J. Biol. Chem.
290, 6408 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
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![[pSer139]Histone H2AX monoclonal antibody (9F3) Western blot](https://www.enzolifesciences.com/fileadmin/thumbs/0774b56b7d06ed0a97549fe8536e019c6852c91c-ADI-KAM-CC255_WB.jpg.jpg "[pSer139]Histone H2AX monoclonal antibody (9F3) Western blot")
