[pSer⁷¹]Vimentin monoclonal antibody (TM71)

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Rabbit anti-rat IgG, polyclonal antibody (HRP conjugate)

ELISA, IHC, WB | Print as PDF

ADI-SAB-200-J

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ADI-KAM-CC250-E	100 µg	402.00 USD
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Alternative Name:	VIM

Clone:	TM71

Host:	Rat

Isotype:	IgG2a

Immunogen:	Synthetic peptide corresponding to aa 66-77 of human Vimentin phosphorylated at Ser⁷¹.

UniProt ID:	P08670

Species reactivity:	Human, Mouse, Rat

Applications:	ICC, WB

Recommended Dilutions/Conditions:	Immunocytochemistry (1µg/ml) Western Blot (1µg/ml for chemiluminescence detection system) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~58kDa by Western blot.

Purity Detail:	Protein G affinity purified.

Formulation:	Liquid. In PBS, pH 7.2, containing 50% glycerol.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Vimentin is one of four type II intermediate filament (IF) proteins; Desmin, Glial fibrillary acidic protein (GFAP) and Peripherin being the others. Vimentin is the most widely distributed of all IF proteins. It is typically expressed in leukocytes, blood vessel endothelial cells, and mesenchymal cells such as fibroblasts. Vimentin filaments help support cellular membranes. Vimentin networks also may help keep the nucleus and other organelles in a defined place within the cell.

Regulatory Status:	RUO - Research Use Only

Western blot analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71).

Immunocytochemistry analysis of phosphorylated vimentin (Ser71) on 4% PFA fixed U251 cells with [pSer71]Vimentin, mAb (TM71).

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