Product Details
Alternative Name: | VIM |
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Clone: | 4A4 |
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Host: | Mouse |
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Isotype: | IgG2b |
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Immunogen: | Synthetic peptide corresponding to a portion of mouse vimentin phosphorylated at Ser55. |
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UniProt ID: | P20152 |
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Species reactivity: | Human, Mouse, Rat
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Applications: | ELISA, ICC, WB
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Recommended Dilutions/Conditions: | ELISA, Immunocytochemistry (1µg/ml) Western Blot (1-5µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~57kDa by Western blot. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Vimentin is one of four type II intermediate filament (IF) proteins; Desmin, Glial fibrillary acidic protein (GFAP) and Peripherin being the others. Vimentin is the most widely distributed of all IF proteins. It is typically expressed in leukocytes, blood vessel endothelial cells, and mesenchymal cells such as fibroblasts. Vimentin filaments help support cellular membranes. Vimentin networks also may help keep the nucleus and other organelles in a defined place within the cell. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of U251 interphase (1) and U251 M phase (2) cells, probed with Vimentin (pSer55) mAb (4A4).
Immunocytochemistry analysis of U251 cells using Vimentin (pSer55) mAb (4A4).
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Product Literature References
Robust elimination of genome-damaged cells safeguards against brain somatic aneuploidy following Knl1 deletion: L. Shi, et al.; Nat. Commun.
10, 2588 (2019),
Abstract;
Full Text
Downregulation of Ribosome Biogenesis During Early Forebrain Development: K.F. Chau, et al.; Elife
7, e36998 (2018),
Abstract;
Full Text
Nuclear pore disassembly from endoplasmic reticulum membranes promotes Ca2+ signalling competency: M.J. Boulware, et al.; J. Physiol.
586, 2873 (2008),
Abstract;
Full Text
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