[pSer⁵⁵]Vimentin monoclonal antibody (4A4)

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ADI-KAM-CC249-E	100 µg	389.00 USD
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Alternative Name:	VIM

Clone:	4A4

Host:	Mouse

Isotype:	IgG2b

Immunogen:	Synthetic peptide corresponding to a portion of mouse vimentin phosphorylated at Ser⁵⁵.

UniProt ID:	P20152

Species reactivity:	Human, Mouse, Rat

Applications:	ELISA, ICC, WB

Recommended Dilutions/Conditions:	ELISA, Immunocytochemistry (1µg/ml) Western Blot (1-5µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~57kDa by Western blot.

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Vimentin is one of four type II intermediate filament (IF) proteins; Desmin, Glial fibrillary acidic protein (GFAP) and Peripherin being the others. Vimentin is the most widely distributed of all IF proteins. It is typically expressed in leukocytes, blood vessel endothelial cells, and mesenchymal cells such as fibroblasts. Vimentin filaments help support cellular membranes. Vimentin networks also may help keep the nucleus and other organelles in a defined place within the cell.

Regulatory Status:	RUO - Research Use Only

[pSer55]Vimentin monoclonal antibody (4A4) Western blot

Western blot analysis of U251 interphase (1) and U251 M phase (2) cells, probed with Vimentin (pSer55) mAb (4A4).

[pSer55]Vimentin monoclonal antibody (4A4) Immunocytochemistry

Immunocytochemistry analysis of U251 cells using Vimentin (pSer55) mAb (4A4).

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ELISA, IHC, WB | Print as PDF

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