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DNA-topoisomerase II α monoclonal antibody (1C5)

ADI-KAM-CC210-E 100 µg 414.00 USD
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Product Details

Immunogen:Recombinant human DNA-topoisomerase IIα (C-terminal fragment).
UniProt ID:P11388
Species reactivity:Human
Applications:Flow Cytometry, ICC, IHC, IP, WB
Recommended Dilutions/Conditions:Flow Cytometry (0.1-1.0µg/ml)
Immunocytochemistry (1-10µg/ml)
Immunohistochemistry (1-5µg/ml)
Immunoprecipitation (1-10µg/ml, 200-300ml cell extract)
Western Blot (0.1-0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of 170kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:DNA-topoisomerase II, also known as Topo II or Top 2, is a multifunctional nuclear enzyme required during DNA replication, chromosome disjunction at mitosis, and other DNA-related activities because of its ability to alter DNA supercoiling. In mammalian cells, Topo II consists of two isozymes, Topo II-alpha (170kDa) and Topo II-beta (180kDa) and expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo II-beta is expressed constitutively throughout the cell cycle, whereas the expression of Topo II-alpha is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. DNA-topoisomerase II is the target of some of the most active drugs used in chemotherapy and has been shown to have a critical role in neural development.
Regulatory Status:RUO - Research Use Only
DNA-topoisomerase II α monoclonal antibody (1C5) Flow Cytometry
Flow cytometry analysis of DNA-Topoisomerase IIa expression in Jurkat. Open histogram indicates reaction of isotypic control to the cells. Shaded histogram indicates the reaction of DNA-Topoisomerase IIa to the cells.
DNA-topoisomerase II α monoclonal antibody (1C5) Immunohistochemistry
Immunohistochemistry analysis of nuclear staining of colon cancer section using DNA-Topoisomerase IIa mAb (1C5)
DNA-topoisomerase II α monoclonal antibody (1C5) Western blot
Western blot analysis of Jurkat cell lysate, probed with DNA-Topoisomerase IIa mAb (1C5)
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DNA-topoisomerase II α monoclonal antibody (1C5) Flow Cytometry DNA-topoisomerase II α monoclonal antibody (1C5) Immunohistochemistry DNA-topoisomerase II α monoclonal antibody (1C5) Western blot

Product Literature References

Securin-independent regulation of separase by checkpoint-induced shugoshin–MAD2: S. Hellmuth, et al.; Nature 580, 536 (2020), Application(s): Western blot, Abstract;
Separase-triggered apoptosis enforces minimal length of mitosis: S. Hellmuth & O. Stemmann; Nature 580, 542 (2020), Application(s): Western blot, Abstract;
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;

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