| ADI-KAM-CC195-E | 100 µg | 343.00 USD |  |
||
| Do you need bulk/larger quantities? | |||||
| Alternative Name: | CCNB1 |
| Clone: | V63 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to the sequence near the carboxy terminus of hamster cyclin B1. |
| UniProt ID: | Q08301 (Chinese hamster) |
| Source: | Purified from mouse ascites. |
| Species reactivity: | Human, Mouse, Rat Dog, Guinea pig, Hamster, Porcine, Rabbit, Sheep |
| Applications: | IHC, WB |
| Recommended Dilutions/Conditions: | Western Blot (1µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of ~58kDa by Western blot. |
| Purity Detail: | Protein G affinity purified. |
| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14-Thr and 15-Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14-Thr and 15-Tyr of p34cdc2 rendering the complex active at the G2/Mboundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Therefore cyclin B-p34cdc2 plays a critical role in G2 to M transition. |
| Regulatory Status: | RUO - Research Use Only |
