Alternative Name: | CCNB1 |
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Clone: | V63 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to the sequence near the carboxy terminus of hamster cyclin B1. |
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UniProt ID: | Q08301 (Chinese hamster) |
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Source: | Purified from mouse ascites. |
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Species reactivity: | Human, Mouse, Rat Dog, Guinea pig, Hamster, Porcine, Rabbit, Sheep
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Applications: | IHC, WB
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Recommended Dilutions/Conditions: | Western Blot (1µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~58kDa by Western blot. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14-Thr and 15-Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14-Thr and 15-Tyr of p34cdc2 rendering the complex active at the G2/Mboundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Therefore cyclin B-p34cdc2 plays a critical role in G2 to M transition. |
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Regulatory Status: | RUO - Research Use Only |
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