| Alternative Name: | CUL1 |
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| Clone: | AS97.1 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant human cullin 1. |
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| UniProt ID: | Q13616 |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Monkey, Rabbit
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:250, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~93kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 0.1mM PMSF and 50% glycerol. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The human Cullin family of genes consists of Cul-1, Cul-2, Cul-3, Cul-4a, Cul-4b, and Cul-5. Among the 6 members, Cul-1 and Cul-2 are very well characterized. Cul-2 is homologous to the Saccharomyces cerevisiae cdc53 gene. Studies have shown that Cul-1 forms an E3 ubiquitin-ligase, called the SCF complex (Skp1 and F-box protein such as bTrCp or FWD1), which controls the proteolysis of G1 cell cycle regulatory proteins. |
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| Regulatory Status: | RUO - Research Use Only |
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