| ADI-KAM-CC111-E | 100 µg | 274.00 USD |  |
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| Alternative Name: | Checkpoint kinase 1 |
| Clone: | DCS-310 |
| Host: | Mouse |
| Isotype: | IgG3 |
| Immunogen: | Recombinant human Chk1. |
| UniProt ID: | O14757 |
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC, IP, WB |
| Recommended Dilutions/Conditions: | Immunohistochemistry (1µg/ml) Immunoprecipitation (2µg/200µl cell extract, 5x106 cells) Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of ~55kDa by Western blot. |
| Purity Detail: | Protein A affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | The DNA damage checkpoint is a signal transduction pathway that has the ability to delay entry into mitosis. When DNA is damaged, the Check point kinases (CHKs) are known to act downstream of ATM to elicit appropriate responses such as cell cycle arrest. Chk1, for instance, is activated by ATM phosphorylation of serines 123, 178, 278, and 292 of the S phase-promoting cdc25A phosphatase and accelerates IR (ionizing radiation)induced degradation of cdc25A. |
| Regulatory Status: | RUO - Research Use Only |
