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Cdk1/cdc2 monoclonal antibody (5F6)

ADI-KAM-CC101-E 100 µg 386.00 USD
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Product Details

Alternative Name:Cyclin-dependent kinase 1, p34cdc2, Cell division cycle 2
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of human Cdk1 (cdc2).
UniProt ID:P06493
Species reactivity:Human, Mouse, Rat
Applications:IHC, IP, WB
Recommended Dilutions/Conditions:Immunohistochemistry (5µg/ml)
Immunoprecipitation (2µg/200µl)
Western Blot (1-5µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~34kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:The cell division cycle (cdc) group of proteins consists of many protein classes, including cyclin dependent kinases (Cdks), phosphoprotein phosphatases, mitogen activated kinases, small GTPases, and cyclins. These proteins play a central role in regulating progression through the cell cycle through the formation and/or modification of key substrates. Cdc42 belongs to the Rho-family of GTPases, and promotes actin polymerization to form filopodia. Cdc25A is a dual-specificity phosphatase that acts on phosphorylated threonine and tyrosine residues of Cdks, leading to binding and activation of their partner cyclins.
Regulatory Status:RUO - Research Use Only
Cdk1/cdc2 monoclonal antibody (5F6) Immunohistochemistry
Immunohistochemistry analysis of paraffin embedded section of rat testis using Cdk1/cdc2 mAb (5F6).
Cdk1/cdc2 monoclonal antibody (5F6) Immunoprecipitation
Immunoprecipitation analysis of Cdk1/cdc2 from A431 cells extract with normal mouse IgG (1) or Cdk1/cdc2 mAb (5F6) (2). After immunoprecipitation with the antibody, the immunocomplex was resolved by SDS-PAGE and immunoBlotted with Cdk1/cdc2 mAb (5F6). A431 crude lysate was resolved in lane 3.
Cdk1/cdc2 monoclonal antibody (5F6) Western blot
Western blot analysis of human HeLa cell lysate probed with Cdk1/cdc2 mAb (5F6).
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Cdk1/cdc2 monoclonal antibody (5F6) Immunohistochemistry Cdk1/cdc2 monoclonal antibody (5F6) Immunoprecipitation Cdk1/cdc2 monoclonal antibody (5F6) Western blot

Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;

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