Product Details
| Alternative Name: | Centromere protein A |
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| Clone: | 3-19 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Synthetic peptide corresponding to a portion of human CENP-A . |
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| UniProt ID: | P49450 |
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| Species reactivity: | Human
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| Applications: | ICC, IHC, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (10µg/ml)
Immunohistochemistry (5µg/ml)
Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects bands of ~18kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Centromere protein A (CENP-A), a 17-19 kDa centromere-specific histone variant, is 62% identical to the carboxy-terminal domain of histone H3. In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A, and H2B. CENP-A defines active centromere regions by forming centromere-specific nucleosomes on which kinetochores are assembled. CENP-A resides specifically in the inner plate of the kinetochore, and is essential for kinetochore targeting of CENP-C and other kinetochore components. CENP-A is required for nucleosomal packaging of centromeric DNA at interphase. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Jurkat (1), Raji (2), PC12 (3), Rat-1 (4), WR19L (5), C2C12 (6), and NIH3T3 (7) cell lysates probed with CENP-A mAb (3-19).
Immunohistochemical detection of CENP-A on paraffin embedded section of a squamous epithelium of human tonsil.
Immunocytochemical detection of CENP-A on acetone fixed HEp-II with CENP-A mAb (3-19).
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Product Literature References
A mitotic chromatin phase transition prevents perforation by microtubules: M.W.G. Schneider, et al.; Nature
609, 183 (2022),
Abstract;
Gene replacement strategies validate the use of functional tags on centromeric chromatin and invalidate an essential role for CENP-AK124ub: C. Salinas-Luypaert, et al.; Cell Rep.
37, 10924 (2021),
Abstract;
Human Artificial Chromosomes that Bypass Centromeric DNA: G.A. Logsdon, et al.; Cell
178, 624 (2019),
Abstract;
Full Text
Phosphorylation of CENP-A on serine 7 does not control centromere function: V. Barra, et al.; Nat. Commun.
10, 175 (2019),
Abstract;
Full Text
CENP-A Modifications on Ser68 and Lys124 Are Dispensable for Establishment, Maintenance, and Long-Term Function of Human Centromeres: D. Fachinetti, et al.; Dev. Cell
40, 104 (2017),
Abstract;
Full Text
Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition: L.Y. Guo, et al.; Nat. Commun.
8, 15775 (2017),
Abstract;
Full Text
Sequences associated with centromere competency in the human genome: K.E. Hayden, et al.; Mol. Cell. Biol.
33, 763 (2013),
Abstract;
Full Text
Composition and organization of active centromere sequences in complex genomes: K.E. Hayden, et al.; BMC Genomics
13, 324 (2012),
Abstract;
Full Text
