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CENP-A monoclonal antibody (3-19)

ADI-KAM-CC006-E 100 µg 414.00 USD
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Product Details

Alternative Name:Centromere protein A
Immunogen:Synthetic peptide corresponding to a portion of human CENP-A .
UniProt ID:P49450
Species reactivity:Human
Applications:ICC, IHC, WB
Recommended Dilutions/Conditions:Immunocytochemistry (10µg/ml)
Immunohistochemistry (5µg/ml)
Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects bands of ~18kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Centromere protein A (CENP-A), a 17-19 kDa centromere-specific histone variant, is 62% identical to the carboxy-terminal domain of histone H3.  In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A, and H2B.  CENP-A defines active centromere regions by forming centromere-specific nucleosomes on which kinetochores are assembled.  CENP-A resides specifically in the inner plate of the kinetochore, and is essential for kinetochore targeting of CENP-C and other kinetochore components.  CENP-A is required for nucleosomal packaging of centromeric DNA at interphase.
Regulatory Status:RUO - Research Use Only
CENP-A monoclonal antibody (3-19) Western blot
Western blot analysis of Jurkat (1), Raji (2), PC12 (3), Rat-1 (4), WR19L (5), C2C12 (6), and NIH3T3 (7) cell lysates probed with CENP-A mAb (3-19).
CENP-A monoclonal antibody (3-19) Immunohistochemistry
Immunohistochemical detection of CENP-A on paraffin embedded section of a squamous epithelium of human tonsil.
CENP-A monoclonal antibody (3-19) ICC
Immunocytochemical detection of CENP-A on acetone fixed HEp-II with CENP-A mAb (3-19).
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CENP-A monoclonal antibody (3-19) Western blot CENP-A monoclonal antibody (3-19) Immunohistochemistry CENP-A monoclonal antibody (3-19) ICC

Product Literature References

A mitotic chromatin phase transition prevents perforation by microtubules: M.W.G. Schneider, et al.; Nature 609, 183 (2022), Abstract;
CENP-A Subnuclear Localization Pattern as Marker Predicting Curability by Chemoradiation Therapy for Locally Advanced Head and Neck Cancer Patients: P. Verrelle, et al.; Cancers (Basel) 13, 3928 (2021), Abstract;
Gene replacement strategies validate the use of functional tags on centromeric chromatin and invalidate an essential role for CENP-AK124ub: C. Salinas-Luypaert, et al.; Cell Rep. 37, 10924 (2021), Abstract;
Subcellular Euclidean distance measurements with multicolor fluorescence localization imaging in cultured cells: T.E. Germanova, et al.; STAR Protoc. 2, 100774 (2021), Abstract;
The structure, function and evolution of a complete human chromosome 8: G.A. Logsdon, et al.; Nature 593, 101 (2021), Abstract;
A genetic memory initiates the epigenetic loop necessary to preserve centromere position: S. Hoffmann, et al.; EMBO J. 39, e105505 (2020), Abstract;
Ensemble-Level Organization of Human Kinetochores and Evidence for Distinct Tension and Attachment Sensors: E. Roscioli, et al.; Cell Rep. 31, 107535 (2020), Abstract;
Human Artificial Chromosomes that Bypass Centromeric DNA: G.A. Logsdon, et al.; Cell 178, 624 (2019), Abstract; Full Text
Phosphorylation of CENP-A on serine 7 does not control centromere function: V. Barra, et al.; Nat. Commun. 10, 175 (2019), Abstract; Full Text
CENP-A Modifications on Ser68 and Lys124 Are Dispensable for Establishment, Maintenance, and Long-Term Function of Human Centromeres: D. Fachinetti, et al.; Dev. Cell 40, 104 (2017), Abstract; Full Text
Centromeres are maintained by fastening CENP-A to DNA and directing an arginine anchor-dependent nucleosome transition: L.Y. Guo, et al.; Nat. Commun. 8, 15775 (2017), Abstract; Full Text
Sequences associated with centromere competency in the human genome: K.E. Hayden, et al.; Mol. Cell. Biol. 33, 763 (2013), Abstract; Full Text
Composition and organization of active centromere sequences in complex genomes: K.E. Hayden, et al.; BMC Genomics 13, 324 (2012), Abstract; Full Text

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