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HO-1 (rat), ELISA kit

Highly sensitive colorimetric immunometric enzyme immunoassay kit with results in < 3 hours
ADI-EKS-810A 96 wells Inquire for pricing
Do you need bulk/larger quantities?
  • Thoroughly validated in serum, plasma, cell lysates, and tissue extracts
  • Highly sensitive quantification of HO-1 surpass semi-quantitative Western blot results
  • Accurate and reproducible results in less than 3 hours
The HO-1 (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Details

Alternative Name:Heme oxygenase 1
Sensitivity:0.036 ng/ml (range 0.195 - 12.5 ng/ml)
Assay Time:<3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of rat HO-1 in cell lysates, plasma, serum, and tissue. Cited sample type includes bronchoalveolar lavage fluid.
Wavelength:450 nm
Species reactivity:Rat
Use/Stability:Store all components at 4°C, except standard at -20°C.
Shipping:Dry Ice and Blue Ice
Contents:Microtiter plate, 5X Extraction reagent 2, Standard, Sample diluent, Wash buffer concentrate, Antibody, Conjugate, TMB Substrate, Stop solution 2
Scientific Background:Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
UniProt ID:P06762
GenBank ID:J02722
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Ambroxol, a mucolytic agent, boosts HO-1, suppresses NF-κB, and decreases the susceptibility of the inflamed rat colon to apoptosis: A new treatment option for treating ulcerative colitis: S. Cavalu, et al.; FASEB J. 36, e22496 (2022), Abstract;
Calycosin alleviates hyperbilirubin nerve injury in Ugt1-/- mice by inhibiting oxidative stress, apoptosis, and mitochondrial function: J. Li, et al.; Acta Histochem. 124, 151918 (2022), Abstract;
Carbocisteine as a Modulator of Nrf2/HO-1 and NFκB Interplay in Rats: New Inspiration for the Revival of an Old Drug for Treating Ulcerative Colitis: A.M. Abdelhamid, et al.; Front. Pharmacol. 13, 887233 (2022), Abstract;
Carvacrol mitigates vancomycin-induced nephrotoxicity via regulation of IkBα/p38MAPK and Keap1/Nrf2 signaling pathways: an experimental study with in silico evidence: M.M. Khalaf, et al.; Eur. Rev. Med. Pharmacol. Sci. 26, 8738 (2022), Abstract;
Heme oxygenase-1 induction mitigates burn-associated early acute kidney injury via the TLR4 signaling pathway: S. Guo, et al.; Burns 48, 156 (2022), Abstract;
Identification of Phytogenic Compounds with Antioxidant Action That Protect Porcine Intestinal Epithelial Cells from Hydrogen Peroxide Induced Oxidative Damage: J. Wang, et al.; Antioxidants 11, 2134 (2022), Abstract;
Omarigliptin attenuates rotenone-induced Parkinson's disease in rats: Possible role of oxidative stress, endoplasmic reticulum stress and immune modulation: H.E. Michel, et al.; Food Chem. Toxicol. 164, 113015 (2022), Abstract;
Ameliorate impacts of scopoletin against vancomycin-induced intoxication in rat model through modulation of Keap1-Nrf2/HO-1 and IκBα-P65 NF-κB/P38 MAPK signaling pathways: Molecular study, molecular docking evidence and network pharmacology analysis: M.M. Khalaf, et al.; Int. Immunopharmacol. 102, 108382 (2021), Abstract;
The immunomodulatory effects of topiramate on azoxymethane-induced colon carcinogenesis in rats: The role of the inflammatory cascade, vascular endothelial growth factor, AKT/mTOR/MAP kinase signaling and the apoptotic markers: A.M. Kabel, et al.; Int. Immunopharmacol. 98, 107830 (2021), Abstract;
Bone marrow–derived mesenchymal stromal cells ameliorate severe acute pancreatitis in rats via hemeoxygenase-1–mediated anti-oxidant and anti-inflammatory: Z. Ma, et al.; Cytotherapy 21, 162 (2019), Application(s): ELISA using rat serum, Abstract;
Usefulness of myeloperoxidase as a biomarker for the ranking of pulmonary toxicity of nanomaterials: T. Tomonaga, et al.; Part. Fibre Toxicol. 15, 41 (2018), Abstract; Full Text
Assessment of Pulmonary Toxicity Induced by Inhaled Toner with External Additives: T. Tomonaga, et al.; Biomed. Res. Int. 2017, 4245309 (2017), Abstract; Full Text
Renoprotective effects of montelukast in an experimental model of cisplatin nephrotoxicity in rats: A.M. Gad, et al.; J. Biochem. Mol. Toxicol. 31, (2017), Application(s): Rat renal lysates, Abstract;
BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats: A. Kowalczyk, et al.; Oxid. Med. Cell. Longev. 2013, 2356853 (2016), Application(s): Rat skeletal muscle hogobenate, Abstract; Full Text
Can heat shock protein 32 be used for the early diagnosis of acute mesenteric ischemia?: S. Berhuni, et al.; Ulus. Cerrahi. Derg. 32, 1 (2016), Application(s): Quantified heme oxygenase (HO) -1 concentrations, Abstract; Full Text
Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung: M. Horie, et al.; Antioxidants (Basel) 5, 4 (2016), Application(s): Rat lung tissue lysates, Abstract; Full Text
Effects of heme oxygenase-1 in the intestine on the intestinal barrier function of rats with hemorrhagic shock: X.Y. Gao, et al.; Int. J. Clin. Exp. Med. 9, 17367 (2016), Application(s): Measurement of HO-1 in rat intestine, Abstract; Full Text
Evaluation of Pulmonary Toxicity of Zinc Oxide Nanoparticles Following Inhalation and Intratracheal Instillation: Y. Morimoto, et al.; Int. J. Mol. Sci. 17, 1241 (2016), Application(s): Concentrations of rat HO-1 in BALF supernatant measurement, Abstract; Full Text
The DPP4 Inhibitor Linagliptin Protects from Experimental Diabetic Retinopathy: N. Dietrich, et al.; PLoS One 11, e0167853 (2016), Abstract;
A pharmacological investigation of Hippophae salicifolia (HS) and Hippophae rhamnoides turkestanica (HRT) against multiple stress (C-H-R): an experimental study using rat model: R. Rathor, et al.; Cell Stress Chaperones 20, 821 (2015), Application(s): rat liver, muscle and heart homogenates, Abstract; Full Text
Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats: A. Fernandez-Bustamante, et al.; PLoS One 10, e0130764 (2015), Abstract; Full Text
FGF9-induced changes in cellular redox status and HO-1 upregulation are FGFR-dependent and proceed through both ERK and AKT to induce CREB and Nrf2 activation: J.I. Chuang, et al.; Free Radic. Biol. Med. 89, 274 (2015), Application(s): Western Blot, Abstract;
Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway: F. Han, et al.; Cell. Physiol. Biochem. 36, 1480 (2015), Application(s): ELISA using rat aortic homogenates , Abstract; Full Text
Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis: H. Anand, et al.; Andrologia 47, 194 (2015), Application(s): Rat testes homogenates, Abstract;
Pulmonary toxicity of well-dispersed cerium oxide nanoparticles following intratracheal instillation and inhalation: Y. Morimoto, et al.; J. Nanopart. Res. 17, 442 (2015), Abstract; Full Text
Pulmonary toxicity of well-dispersed titanium dioxide nanoparticles following intratracheal instillation: Y. Yoshiura, et al.; J. Nanopart. Res. 17, 241 (2015), Abstract; Full Text
Recovery of redox homeostasis altered by CuNPs in H4IIE liver cells does not reduce the cytotoxic effects of these NPs: An investigation using aryl hydrocarbon receptor (AhR) dependent antioxidant activity: M. Connolly, et al.; Chem. Biol. Interact. 228, 57 (2015), Abstract;
The molecular fingerprint of lung inflammation after blunt chest trauma: C. Ehrntaller, et al.; Eur. J. Med. Res. 20, 70 (2015), Abstract; Full Text
Ellagic acid antiinflammatory and antiapoptotic potential mediate renoprotection in cisplatin nephrotoxic rats: A.M. El-Garhy, et al.; J. Biochem. Mol. Toxicol. 28, 472 (2014), Application(s): Rat renal lysates, Abstract;
Heme oxygenase-1 induction by cobalt protoporphyrin enhances fever and inhibits pyrogenic tolerance to lipopolysaccharide: J. Piotrowski, et al.; J. Therm. Biol. 45, 69 (2014), Application(s): Rat blood plasma, Abstract;
The Heme Oxygenase System Suppresses Perirenal Visceral Adiposity, Abates Renal Inflammation and Ameliorates Diabetic Nephropathy in Zucker Diabetic Fatty Rats: J.F. Ndisang, et al.; PLoS One 9, e87936 (2014), Abstract; Full Text
Pulmonary toxicity of printer toner following inhalation and intratracheal instillation: Y. Morimoto, et al.; Inhal. Toxicol. 25, 679 (2013), Application(s): ELISA using rat bronchoalveolar lavage fluid, Abstract;
Upregulation of cytoprotective defense mechanisms and hypoxia-responsive proteins imparts tolerance to acute hypobaric hypoxia: K. Jain, et al.; High Alt. Med. Biol. 14, 65 (2013), Application(s): ELISA using rat heart tissue homogenate, Abstract;
Pulmonary inflammation of well-dispersed multi-wall carbon nanotubes following intratracheal instillation: M. Horie, et al; Materials 5, 2833 (2012), Application(s): ELISA using rat bronchoalveolar lavage fluid, Full Text
Ischemic preconditioning and remote ischemic preconditioning have protective effect against cold ischemia–reperfusion injury of rat small intestine: I. Saeki, et al.; Pediatr. Surg. Int. 27, 857 (2011), Application(s): ELISA using rat serum, Abstract;
4-Methylcatechol-induced heme oxygenase-1 exerts a protective effect against oxidative stress in cultured neural stem/progenitor cells via PI3 kinase/Akt pathway: Y. Furukawa, et al.; Biomed. Res. 31, 45 (2010), Application(s): ELISA using rat cell extracts, Abstract; Full Text
Up-regulation of heme oxygenase-1 in rat spleen after aniline exposure: J. Wang, et al.; Free Radic. Biol. Med. 48, 513 (2010), Application(s): ELISA using rat spleen tissue lysates, Abstract; Full Text

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