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HO-1 (human), ELISA kit

Highly sensitive HO-1 ELISA kit enabling detection of HO-1 in cell lysates and tissue extracts in < 2.5 hours.
ADI-EKS-800 96 wells 664.00 USD
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  • Highly sensitive assay, measure as little as 0.78 ng/ml of HO-1
  • Reliable and reproducible results day-after-day and lot-after-lot
  • Higher throughput format, assay up to 40 samples in duplicate in just 2.5 hours
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The HO-1 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 2.5 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Specification

Alternative Name:Heme oxygenase 1
Sensitivity:0.78 ng/ml (range 0.78 - 25 ng/ml)
Assay Time:<2.5 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of human heme oxygenase-1 in cell lysates and tissue extracts. Cited sample types include serum and plasma.
Species reactivity:Human
Crossreactivity:Human HO-1 (100%). No reactivity with HO-2 or HO-3.
Use/Stability:Store all components at 4°C, except standard at -20°C.
Shipping:Shipped on Blue Ice
Contents:Microtiter plate, 5X Extraction reagent, Standard, Sample diluent, Wash buffer concentrate, Antibody, Antibody diluent, HRP Conjugate, Conjugate diluent, TMB Substrate, Stop solution 2
Scientific Background:Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
UniProt ID:P09601
Regulatory Status:RUO - Research Use Only

Product Literature References

Beneficial use of serum ferritin and heme oxygenase-1 as biomarkers in adult-onset Still's disease: A multicenter retrospective study: Y. Kirino, et al.; Mod. Rheumatol. 28, 858 (2018), Application(s): ELISA using human serum, Abstract;
Camel Milk Ameliorates 5-Fluorouracil-Induced Renal Injury in Rats: Targeting MAPKs, NF-κB and PI3K/Akt/eNOS Pathways: H.H. Arab, et al.; Cell. Physiol. Biochem. 46, 1628 (2018), Application(s): ELISA with Rat, Abstract; Full Text
ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome: Y. Hara, et al.; Can. Respir. J. 2018, 9627420 (2018), Application(s): Human serum, Abstract; Full Text
Endothelin-1-induced hypertrophic alterations and heme oxygenase-1 expression in cardiomyoblasts are counteracted by beta estradiol: in vitro and in vivo studies: T. Barta, et al.; Naunyn Schmiedebergs Arch. Pharmacol. 391, 371 (2018), Abstract; Full Text
Haemolysis and haem oxygenase-1 induction during persistent "asymptomatic" malaria infection in Burkinabé children: J.P. Mooney, et al.; Malar. J. 17, 253 (2018), Abstract; Full Text
Hepatoprotective Potential of Standardized Ficus Species in Intrahepatic Cholestasis Rat Model: Involvement of Nuclear Factor-κB, and Farnesoid X Receptor Signaling Pathways: S.S. El-Hawary, et al.; J. Ethnopharmacol. 231, 262 (2018), Abstract;
Lutzomyia longipalpis Saliva Induces Heme Oxygenase-1 Expression at Bite Sites: N.F. Luz, et al.; Front. Immunol. 9, 2779 (2018), Application(s): Human serum, Abstract; Full Text
Nicorandil ameliorates pulmonary inflammation and fibrosis in a rat model of silicosis: D.H. El-Kashef, et al.; Int. Immunopharmacol. 64, 289 (2018), Abstract;
Paradoxical cardiotoxicity of intraperitoneally-injected epigallocatechin gallate preparation in diabetic mice: N.O.A. Rasheed, et al.; Sci. Rep. 8, 7880 (2018), Abstract; Full Text
Plasma Heme Oxygenase-1 Levels and Carotid Atherosclerosis: Y. Kishimoto, et al.; Stroke 49, 2230 (2018), Application(s): Human plasma, Abstract;
Relationship between Anaemia, Haemolysis, Inflammation and Haem Oxygenase-1 at Admission with Sepsis: a pilot study: P. Ekregbesi, et al.; Sci. Rep. 8, 11198 (2018), Abstract; Full Text
Simvastatin treatment upregulates HO-1 in patients with abdominal aortic aneurysm but independently of Nrf2: A. Piechota-Polanczyk, et al.; Oxid. Med. Cell. Longev. 2018, 2028936 (2018), Application(s): ELISA using tissue extracts, Abstract; Full Text
The hemoglobin degradation pathway in patients with preeclampsia – Fetal hemoglobin, heme, heme oxygenase-1 and hemopexin – Potential diagnostic biomarkers?: U.D. Anderson, et al.; Pregnancy Hypertens. 14, 273 (2018), Application(s): Human Plasma, Abstract;
Astrocyte heme oxygenase-1 reduces mortality and improves outcome after collagenase-induced intracerebral hemorrhage: J. Chen-Roetling, et al.; Neurobiol. Dis. 102, 140 (2017), Application(s): Overexpression of human HO-1 in transgenic mouse striata, Abstract; Full Text
Combined Delivery of a Lipopolysaccharide-Binding Peptide and the Heme Oxygenase-1 Gene Using Deoxycholic Acid-Conjugated Polyethylenimine for the Treatment of Acute Lung Injury: J.Y. Kim, et al.; Macromol. Biosci. 17, (2017), Application(s): Mouse lung tissue lysates, Abstract;
Data on HO-1 and CD200 protein secretion during T-cells and mesenchymal stromal cells co-cultures: M. Fayyad-Kazan, et al.; Data Brief 442 (2017), Application(s): Cell lysates of human mesenchymal stromal cells (MSCs), Abstract; Full Text
Deoxycholic Acid-Conjugated Polyethylenimine for Delivery of Heme Oxygenase-1 Gene in Rat Ischemic Stroke Model: J. Oh, et al.; J. Pharm. Sci. 106, 3524 (2017), Application(s): Lysates of mouse Neuro2A cells and rat brain tissue, Abstract;
Mycobacterium tuberculosis Induction of Heme Oxygenase-1 Expression Is Dependent on Oxidative Stress and Reflects Treatment Outcomes: N. Rockwood, et al.; Front. Immunol. 8, 542 (2017), Abstract; Full Text
Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis: U. Wasik, et al.; Sci. Rep. 7, 44769 (2017), Abstract; Full Text
Association of the microsatellite (GT)n repeat polymorphisms of the HO-1 gene promoter and corresponding serum levels with the risk of laryngeal squamous cell carcinoma: D. Tang, et al.; Acta Otolaryngol. 136, 806 (2016), Application(s): Human serum, Abstract;
Cellular Effects in an In Vitro Human 3D Cellular Airway Model and A549/BEAS-2B In Vitro Cell Cultures Following Air Exposure to Cerium Oxide Particles at an Air-Liquid Interface: I.M. Kooter, et al.; Applied in vitro Toxicology 2, 56 (2016), Application(s): Measurement of heme oxygenase (HO-1) protein expression in human cells, Abstract;
Delivery of Hypoxia-Inducible Heme Oxygenase-1 Gene for Site-Specific Gene Therapy in the Ischemic Stroke Animal Model: M. Choi, et al.; Pharm. Res. 33, 2250 (2016), Application(s): Cell lysates of mouse Neuro2A neuroblastoma cells, Abstract;
Erythropoietin Levels Increase during Cerebral Malaria and Correlate with Heme, Interleukin-10 and Tumor Necrosis Factor-Alpha in India: E. Dalko, et al.; PLoS One 11, e0158420 (2016), Application(s): Determination of EPO, total heme, hemopexin and heme oxygenase (HO)-1 levels in plasma, Abstract; Full Text
Heme oxygenase-1 regulates inflammation and mycobacterial survival in human macrophages during Mycobacterium tuberculosis infection: C.R. Scharn, et al.; J. Immunol. 196, 4641 (2016), Abstract;
Identification of CD163 as an antiinflammatory receptor for HMGB1-haptoglobin complexes: H. Yang, et al.; JCI Insight 1, e85375 (2016), Application(s): Human Monocyte THP1 cell lysates, Abstract; Full Text
Iron Supplementation Alters Heme and Heme Oxygenase 1 (HO-1) Levels In Pregnant Women in Ghana: H. Salifu, et al.; SOJ Microbiol. Infect. Dis. 4, (2016), Application(s): Human Plasma, Abstract; Full Text
Microbial Translocation Associated with an Acute-Phase Response and Elevations in MMP-1, HO-1, and Proinflammatory Cytokines in Strongyloides stercoralis Infection: A. Rajamanickam, et al.; Infect. Immun. 85, e00772-16 (2016), Application(s): Human Plasma, Abstract; Full Text
Astrocyte overexpression of heme oxygenase-1 improves outcome after intracerebral hemorrhage: J. Chen-Roetling, et al.; Stroke 46, 1093 (2015), Application(s): HO-1 levels in injected and contralateral striata, Abstract; Full Text
Differential expression of heat shock proteins in fish hepatocytes under hypoxic condition: E. Padmini, et al.; Int. J. Fish. Aquat. Stud. 3, 447 (2015), Application(s): Quantification of HO-1, Full Text
Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytes: E. Padmini & J. Tharani; Environ. Monit. Assess. 187, 4147 (2015), Application(s): Fish (M. cephalus) hepatocyte extracts, Abstract;
Liraglutide Reduces Oxidative Stress And Restores Heme Oxygenase-1 and Ghrelin Levels in Patients with Type 2 Diabetes: A Prospective Pilot Study: M. Rizzo, et al.; J. Clin. Endocrinol. Metab. 100, 603 (2015), Application(s): Human serum, Abstract; Full Text
Mechanisms of Antioxidant Induction with High-Dose N-Acetylcysteine in Childhood Cerebral Adrenoleukodystrophy: R.V. Kartha, et al.; CNS Drugs 29, 1041 (2015), Application(s): Human plasma, Abstract;
Relationship between NOX4 level and angiotensin II signaling in Gitelman’s syndrome. Implications with hypertension: L.A. Calò, et al.; Int. J. Clin. Exp. Med. 8, 7487 (2015), Application(s): ELISA using human cell lysates, Abstract; Full Text
Increased RBP4 in a human model of activated anti-atherosclerotic and antiremodelling defences: L.A. Calò, et al.; Eur. J. Clin. Invest. 44, 567 (2014), Application(s): Protein extract from human PBMCs, Abstract;
Non-coding RNAs and heme oxygenase-1 in vaccinia virus infection: C.A. Meseda, et al.; Biochem. Biophys. Res. Commun. 454, 84 (2014), Application(s): Cell lysates from human monocyte-derived macrophages, Abstract; Full Text
Cytokine profiles in children with primary Epstein-Barr virus infection: T. Wada, et al.; Pediatr. Blood Cancer 60, E46 (2013), Application(s): Human serum or plasma, Abstract;
Increased microRNA-146a levels in plasma of patients with newly diagnosed type 2 diabetes mellitus: Y. Rong, et al.; PLoS One 8, e73272 (2013), Application(s): HO-1 levels in human plasma, Abstract; Full Text
Sour cherry seed kernel extract increases heme oxygenase-1 expression and decreases representation of CD3+ TNF-α+ and CD3+IL-8+ subpopulations in peripheral blood leukocyte cultures from type 2 diabetes patients: F.F. Mahmoud, et al.; Phytother. Res. 27, 767 (2013), Application(s): Cell lysates of primary human leukocytes, Abstract;
Transcription factor Nrf2 protects renal dopamine D1 receptor function during oxidative stress: A.A. Banday, et al.; Hypertension 62, 512 (2013), Application(s): HO-1 levels in renal proximal tubules, Abstract; Full Text
Antioxidant and cytoprotective effects of L-Serine on human endothelial cells: M.N. Maralani, et al.; Res. Pharm. Sci. 7, 209 (2012), Application(s): ELISA using HUVEC cell lysates, Abstract; Full Text
Heme oxygenase microsatellite polymorphism, oxidative stress, glycemic control, and complication development in type 2 diabetes patients: S.W. Choi, et al.; Free Radic. Biol. Med. 53, 60 (2012), Application(s): HO-1 levels in human plasma, Abstract;
Interleukin-19 (IL-19) Induces Heme Oxygenase-1 (HO-1) Expression and Decreases Reactive Oxygen Species in Human Vascular Smooth Muscle Cells: K. Gabina, et al.; J. Biol. Chem. 287, 2477 (2012), Application(s): Cell lysates of primary human coronary artery EC and VSMC, Abstract; Full Text
Maternal Serum Heme-Oxygenase-1 (HO-1) Concentrations in Early Pregnancy and Subsequent Risk of Gestational Diabetes Mellitus: C. Qiu, et al.; PLoS One 7, e48060 (2012), Application(s): Human serum, Abstract; Full Text
Plasma heme oxygenase-1 concentration in relation to impaired glucose regulation in a non-diabetic Chinese population.: W. Bao, et al.; PLoS One 7, e32223 (2012), Abstract; Full Text
Schizophrenia-like features in transgenic mice overexpressing human HO-1 in the astrocytic compartment: W. Song, et al.; J. Neurosci. 8, 32 (2012), Application(s): ELISA using transgenic mouse brain, Abstract; Full Text
Unregulated brain iron deposition in transgenic mice over-expressing HMOX1 in the astrocytic compartment: W. Song, et al.; J. Neurochem. 123, 325 (2012), Application(s): ELISA using transgenic mouse brain, Abstract;
First in-human study demonstrating pharmacological activation of heme oxygenase-1 in human: A. Bharucha, et al.; Clin. Pharmacol. Ther. 87, 187 (2010), Application(s): EIA using human plasma, Abstract;
Increased serum HO-1 in hemophagocytic syndrome and adult-onset Still's disease: use in the differential diagnosis of hyperferritinemia: Y. Kirino, et al.; Arthritis Res. Ther. 7, R616 (2005), Application(s): EIA using human serum, Abstract;
Biliverdin reductase, a novel regulator for induction of activating transcription factor-2 and heme oxygenase-1: M.D. Maines, et al. ; J. Biol. Chem. 279, 19916 (2004), Application(s): EIA using human cell lysates, Abstract;
Hemoglobin scavenger receptor CD163 mediates interleukin-10 release and heme oxygenase-1 synthesis: anti inflammatory monocyte-macrophage responses in vitro, in resolving skin blisters in vivo, and after cardiopulmonary bypass surgery: R.C. Landis, et al. ; Circ. Res. 94, 119 (2004), Application(s): EIA using human cell lysates, Abstract;

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