- Quantitative - fully quantitate levels of auto-antibodies to HSP70
- Higher throughput - obtain results from up to 41 samples in duplicate in less than 4 hours
- Easy-to-use - liquid color-coded reagents and pre-coated plates reduce errors and save time in the lab
This assay allows for reproducible, accurate and precise determination of IgG , IgA and IgM antibodies (total) to human Hsp70 in serum. The ELISA kit uses recombinant human Hsp70 bound to the wells of the plate to bind anti-human Hsp60 antibodies present in human serum. The captured anti-human Hsp70 antibodies are detected with a HRP conjugated goat polyclonal antibody specific for human IgG, IgA and IgM molecules. The assay is developed with tetramethylbenzidine substrate pro ducing a blue color in proportion to the amount of captured anti -human Hsp70 antibodies. The color development is stopped with acid stop solution which converts the endpoint color to yellow. The intensity of the color is measured in a microplate reader at 450 nm.
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Product Details
| Alternative Name: | Heat shock protein 70, HSP72 |
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| Sensitivity: | 6.79 ng/ml (range 31.25 - 1,000 ng/ml) |
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| Assay Time: | <4 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of anti-HSP70 IgG/A/M autoantibodies in human serum. Cited sample types include human plasma as well as rat serum or rat plasma. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | Microtiter plate, Standard, Sample diluent 2, Wash buffer concentrate, HRP Conjugate, TMB Substrate, Stop solution 2 |
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| Scientific Background: | The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Prevalence of immune-mediated inner ear disease in non-segmental vitiligo: A cross-sectional study: H.Z. Marchioro, et al.; Autoimmun. Rev.
22, 103336 (2023),
Abstract;
Intrapulmonary autoantibodies to HSP72 are associated with improved outcomes in IPF: R. Mills, et al.; J. Immunol. Res.
2019, 1845128 (2019),
Application(s): ELISA using human BALF and serum,
Abstract;
Full Text
Heat shock protein 70 and anti-heat shock protein 70 antibodies in patients with chronic glomerulonephritis: N. Chebotareva, et al.; Cell Stress Chaperones
23, 1229 (2018),
Application(s): ELISA using human urine,
Abstract;
Full Text
Heat shock protein 70 and antibodies to heat shock protein 60 are associated with cerebrovascular atherosclerosis: R. Galovic, et al.; Clin. Biochem.
49, 66 (2016),
Application(s): Measurements of serum concentrations of Hsp70,
Abstract;
Decreased serum heat shock protein 60 levels in newly diagnosed immune thrombocytopenia patients: I. Dolasik, et al.; Platelets
26, 220 (2015),
Application(s): ELISA kit using human serum,
Abstract;
Early inflammatory response in polytraumatized patients: Cytokines and heat shock proteins. A pilot study: M.C. Guisasola, et al.; Orthop. Traumatol. Surg. Res.
101, 607 (2015),
Application(s): ELISA using human serum,
Abstract;
Expression of HSPs: an adaptive mechanism during long-term heat stress in goats (Capra hircus): S.S. Dangi, et al.; Int. J. Biometeorol.
59, 1095 (2015),
Abstract;
Modulatory effect of betaine on expression dynamics of HSPs during heat stress acclimation in goat (Capra hircus): S.S. Dangi, et al.; Gene (2015),
Application(s): ELISA measuring HSP70 concentration goat PBMC lysate,
Abstract;
Significance of serum antibodies against HSP 60 and HSP 70 for the diagnostic of infectious diseases: C. Bleotu, et al.; Virulence
5, 828 (2014),
Application(s): ELISA kit using human serum,
Abstract;
Circulating anti-Hsp70 levels in nascent metabolic syndrome: the Casale Monferrato Study: G. Gruden, et al.; Cell. Stress Chaperones
18, 353 (2013),
Application(s): ELISA kit using human serum,
Abstract;
Full Text
Evidence of a role for both anti-Hsp70 antibody and endothelial surface membrane Hsp70 in atherosclerosis: X. Leng, et al.; Cell. Stress Chaperones
18, 483 (2013),
Application(s): ELISA kit using rat plasma,
Abstract;
Full Text
Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats: F.H. Saito, et al.; Cell. Stress Chaperones
18, 25 (2013),
Application(s): ELISA kit using rat serum,
Abstract;
Full Text
High levels of heat shock protein 70 are associated with pro-inflammatory cytokines and may differentiate early- from late-onset preeclampsia: J.C. Peraçoli, et al.; J. Reprod. Immunol.
100, 129 (2013),
Application(s): ELISA kit using human plasma,
Abstract;
Response of circulating heat shock protein 70 and anti-heat shock protein 70 antibodies to catheter ablation of atrial fibrillation: J. Kornej, et al.; J. Transl. Med.
11, 49 (2013),
Application(s): EIA using human plasma samples,
Abstract;
Full Text
Clinical significance of serological biomarkers and neuropsychological performances in patients with temporal lobe epilepsy: C.C. Chang, et al.; BMC Neurol.
12, 15 (2012),
Application(s): ELISA kit using human plasma,
Abstract;
Full Text
Anti-Heat Shock Protein 70 antibody levels are increased in myasthenia gravis and Guillain-Barré syndrome: G. Helgeland, et al.; J. Neuroimmunol.
225, 180 (2010),
Application(s): ELISA kit using human serum,
Abstract;
Plasma levels of Hsp70 and anti-Hsp70 antibody predict risk of acute coronary syndrome: X. Zhang, et al.; Cell. Stress Chaperones
15, 675 (2010),
Application(s): ELISA kit using human plasma,
Abstract;
Full Text
The small heat shock protein 27 is a key regulator of CD8+ CD57+ lymphocyte survival: K. Wood, et al.; J. Immunol.
15, 184 (2010),
Application(s): EIA using human lymphocytes,
Abstract;
Full Text
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