- Quantitative - fully quantitate levels of auto-antibodies to HSP60
- Higher throughput - obtain results from up to 41 samples in duplicate in less than 4 hours
- Easy-to-use - liquid color-coded reagents and pre-coated plates reduce errors and save time in the lab
This assay allows for reproducible, accurate and precise determination of IgG , IgA and IgM antibodies (total) to human Hsp60 in serum. The ELISA kit uses recombinant human Hsp60 bound to the wells of the plate to bind anti-human Hsp60 antibodies present in human serum. The captured anti-human Hsp60 antibodies are detected with a HRP conjugated goat polyclonal antibody specific for human IgG, IgA and IgM molecules. The assay is developed with tetramethylbenzidine substrate pro ducing a blue color in proportion to the amount of captured anti -human Hsp60 antibodies. The color development is stopped with acid stop solution which converts the endpoint color to yellow. The intensity of the color is measured in a microplate reader at 450 nm.
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Product Details
| Sensitivity: | 2.88 ng/ml (range 7.81 - 250 ng/ml) |
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| Assay Time: | <4 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of anti-HSP60 IgG/A/M autoantibodies in human serum. Cited sample types include human plasma. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter plate, Standard, Sample diluent 1, Wash buffer concentrate, HRP Conjugate, TMB Substrate, Stop solution 2 |
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| Scientific Background: | Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor. |
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| UniProt ID: | P10809 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese: A. Khadir, et al.; Front. Endocrinol. (Lausanne)
9, 16 (2018),
Abstract;
Full Text
Antibodies against small heat-shock proteins in Alzheimer’s disease as a part of natural human immune repertoire or activation of humoral response?: E. Papuc, et al.; J. Neural Transm. (Vienna)
123, 455 (2016),
Abstract;
Full Text
Heat shock protein 70 and antibodies to heat shock protein 60 are associated with cerebrovascular atherosclerosis: R. Galovic, et al.; Clin. Biochem.
49, 66 (2016),
Application(s): Measurements of serum concentrations of Hsp60,
Abstract;
A relationship between serological markers of chronic C. pneumoniae and CMV infection and hsp60 in patients with atherosclerotic carotid stenosis: M. Rabczyński, et al.; Acta Biochim. Pol.
62, 89 (2015),
Application(s): ELISA,
Abstract;
Full Text
Decreased serum heat shock protein 60 levels in newly diagnosed immune thrombocytopenia patients: I. Dolasik, et al.; Platelets
26, 220 (2015),
Abstract;
Humoral response against small heat shock proteins in Parkinson's disease.: E. Papuć, et al.; PLoS One
10, e011548 (2015),
Application(s): ELISA using human serum samples,
Abstract;
Humoral Response against Small Heat Shock Proteins in Parkinson’s Disease: E. Papuc, et al.; PLoS One
10, e0115480 (2015),
Abstract;
Full Text
Significance of serum antibodies against HSP 60 and HSP 70 for the diagnostic of infectious diseases: C. Bleotu, et al.; Virulence
5, 828 (2014),
Abstract;
Detection of autoantibodies against heat shock proteins and collapsin response mediator proteins in autoimmune retinopathy: G. Adamus, et al.; BMC Ophthalmol.
13, 48 (2013),
Application(s): ELISA using human serum samples,
Abstract;
Full Text
High levels of heat shock protein 70 are associated with pro-inflammatory cytokines and may differentiate early- from late-onset preeclampsia: J.C. Peraçoli, et al.; J. Reprod. Immunol.
100, 129 (2013),
Application(s): ELISA using human plasma samples,
Abstract;
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