Safe - non-radioactive measurement of kinase activity
Flexible - kinetic and end-point options available
Fast - results in < 4.5 hours
Efficient - only 30 μl diluted sample needed per well
The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
Please mouse over
Product Details
Applications:
Colorimetric detection Activity assay
Application Notes:
For the measurement of p70 S6K activity in partially purified, purified, or crude enzyme preparations from any species.
Species reactivity:
Species independent
Use/Stability:
Store all components at 4°C, except active kinase at -80°C.
p70 S6K is a mitogen stimulated serine/threonine protein kinase that regulates the cell growth and G1 phase cell cycle progression. p70 S6K is activated by serum stimulation and can be inhibited by both wortmannin and rapamycin. Activation of p70 S6K by multi-site phosphorylation requires phosphoinositide 3-kinase (PI3-K)-dependent signal(s) leading to sequential phosphorylation at two key residues: Thr389 and Thr229. Thr229 phosphorylation is mediated by phosphoinositide-dependent kinase 1 (PDK-1), and is dependent upon prior phosphorylation of Thr389. Multiple residues in the p70 S6K pseudosubstrate domain (e.g. Ser411, Thr421, and Ser424) are also subject to activating phosphorylation by upstream kinases.
RAPTOR Controls Developmental Growth Transitions by Altering the Hormonal and Metabolic Balance: M.A. Salem, et al.; Plant Physiol. 177, 565 (2018), Abstract; Full Text
Ribosomal protein S6 kinase1 coordinates with TOR-Raptor2 to regulate thylakoid membrane biosynthesis in rice: L. Sun, et al.; Biochim. Biophys. Acta 1861, 639 (2016), Application(s): Analyzed S6K1 kinase activity, Abstract;
Functional role of RNA polymerase II and P70 S6 kinase in KCl withdrawal-induced cerebellar granule neuron apoptosis: J. Padmanabhan, et al. ; J. Biol. Chem. 290, 5267 (2015), Application(s): Assay, Abstract; Full Text
Hypophosphorylation of ribosomal protein S6 is a molecular mechanism underlying ischemic tolerance induced by either hibernation or preconditioning: S. Miyake, et al.; J. Neurochem. 135, 943 (2015), Abstract; Full Text
Reducing Ribosomal Protein S6 Kinase 1 Expression Improves Spatial Memory and Synaptic Plasticity in a Mouse Model of Alzheimer's Disease: A. Caccamo, et al.; J. Neurosci. 35, 14042 (2015), Abstract; Full Text
