The Rho activation kit provides a simple, convenient and efficient tool for monitoring the activation of the small GTPase, Rho. This assay uses a GST-fusion protein containing the Rho-binding domain (RBD) of mouse Rhotekin to affinity precipitate active Rho (GTP-Rho) from cell lysates. The GST-Rhotekin-RBD fusion protein (~36kDa) is incubated with cell lysate and a glutathione resin. The pulled -down active or GTP-Rho is detected by Western blot analysis using a specific Rho Antibody.
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Product Details
| Applications: | WB
Activity assay, GST pulldown
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| Application Notes: | For the measurement of active Rho in cell lysates of human, mouse, and rat origin. |
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| Species reactivity: | Human, Mouse, Rat
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| Use/Stability: | Reagents require separate storage conditions upon receipt. |
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| Shipping: | Dry Ice |
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| Contents: | Glutathione Resin, 2X SDS Sample Buffer, 1X Lysis/Binding/Wash Buffer, Antibody, GST-Rhotekin-RBD, 100X GDP, 100X GTP, Spin Cups, Collection Tubes |
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| Scientific Background: | The Rho, Rac, and cdc42 subfamilies belong to the Rho-family of small GTP-binding proteins (G proteins or GTPases). Rho proteins have been shown to regulate actin stress fiber formation, focal adhesion and cell migration. Rho has also been shown to stimulate gene transcription and participate in Ras-induced cell transformation. Increased expression of some Rho family members appears to be a frequent event in different types of human tumors, making them potential targets for therapeutic intervention. Like all small GTPases, Rho acts as a switch regulating molecular events by cycling between an inactive GDP-bound state and an active GTP-bound state. |
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| Regulatory Status: | RUO - Research Use Only |
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