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Ras activation kit

ADI-EKS-460 30 tests 1,257.00 USD
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The Ras activation kit provides a simple, convenient and efficient tool for monitoring the activation of the small GTPase, Ras. This assay uses a GST-fusion protein containing the Ras-binding domain (RBD) of Raf1 to affinity precipitate active Ras (GTP-Ras) from cell lysates. The GST-Raf1-RBD fusion protein (~42kDa) is incubated with cell lysate and an Immobilized Glutathione Resin. The pulled-down active or GTP-Ras is detected by Western blot analysis using a specific Ras Antibody.
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Product Details

Activity assay, GST pulldown
Application Notes:For the measurement of active Ras in cell lysates of human, mouse, and rat origin.
Species reactivity:Human, Mouse, Rat
Use/Stability:Reagents require separate storage conditions upon receipt.
Shipping:Shipped on Dry Ice
Contents:Glutathione Resin, 2X SDS Sample Buffer, 1X Lysis/Binding/Wash Buffer, Antibody, GST-Raf1-RBD, 100X GDP, 100X GTP, Spin Cups, Collection Tubes
Scientific Background:Monomeric G-protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to a number of downstream signaling pathways. Oncogenic mutations, which occur in approximately 30% of all cases of human cancer, lead to the stimulation of proliferation and the inhibition of apoptosis.
UniProt ID:P01112
Regulatory Status:RUO - Research Use Only

Product Literature References

Novel β-phenylacrylic acid derivatives exert anti-cancer activity by inducing Src-mediated apoptosis in wild-type KRAS colon cancer: S.J. Kim, et al.; Cell Death Dis. 9, 877 (2018), Abstract; Full Text

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