The Ras activation kit provides a simple, convenient and efficient tool for monitoring the activation of the small GTPase, Ras. This assay uses a GST-fusion protein containing the Ras-binding domain (RBD) of Raf1 to affinity precipitate active Ras (GTP-Ras) from cell lysates. The GST-Raf1-RBD fusion protein (~42kDa) is incubated with cell lysate and an Immobilized Glutathione Resin. The pulled-down active or GTP-Ras is detected by Western blot analysis using a specific Ras Antibody.
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Product Details
| Applications: | WB
Activity assay, GST pulldown
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| Application Notes: | For the measurement of active Ras in cell lysates of human, mouse, and rat origin. |
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| Species reactivity: | Human, Mouse, Rat
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| Use/Stability: | Reagents require separate storage conditions upon receipt. |
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| Shipping: | Shipped on Dry Ice |
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| Contents: | Glutathione Resin, 2X SDS Sample Buffer, 1X Lysis/Binding/Wash Buffer, Antibody, GST-Raf1-RBD, 100X GDP, 100X GTP, Spin Cups, Collection Tubes |
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| Scientific Background: | Monomeric G-protein Ras functions as a molecular switch linking receptor and nonreceptor tyrosine kinase activation to a number of downstream signaling pathways. Oncogenic mutations, which occur in approximately 30% of all cases of human cancer, lead to the stimulation of proliferation and the inhibition of apoptosis. |
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| UniProt ID: | P01112 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Novel β-phenylacrylic acid derivatives exert anti-cancer activity by inducing Src-mediated apoptosis in wild-type KRAS colon cancer: S.J. Kim, et al.; Cell Death Dis.
9, 877 (2018),
Abstract;
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