The Rap1 activation kit provides a simple, convenient and efficient tool for monitoring the activation of the small GTPase, Rap1. This assay uses a GST-fusion protein containing the Rap1 binding domain (RBD) of human Ra1GDS to affinity precipitate active Rap1 (GTP-Rap1) from cell lysates. The GST-Ra1GDS-RBD fusion protein (~40kDa) is incubated with cell lysate and a Glutathione Resin. The pulled-down active or GTP-Rap1 is detected by Western blot analysis using a specific Rap1 Antibody.
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Product Details
| Applications: | WB
Activity assay, GST pulldown
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| Application Notes: | For the measurement of active Rap1 in cell lysates of human, mouse, and rat origin. |
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| Species reactivity: | Human, Mouse, Rat
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| Use/Stability: | Reagents require separate storage conditions upon receipt. |
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| Shipping: | Dry Ice |
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| Contents: | Glutathione Resin, 2X SDS Sample Buffer, 1X Lysis/Binding/Wash Buffer, Antibody, GST-Ra1GDS-RBD, 100X GDP, 100X GTP, Spin Cups, Collection Tubes |
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| Scientific Background: | Rap1, which is a member of the Ras family of GTP-binding proteins, cycles between an active GTP-bound and an unactive GDP-bound form that is mediated by GTPase activating protein (GAP). Rap1 is proposed to regulate Ras-mediated signaling and may also be involved in the regulation of Integrin-mediated cell adhesion although the mechanism of regulation is not known. |
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| UniProt ID: | P62834 (Rap1A), P61224 (Rap1B) |
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| Regulatory Status: | RUO - Research Use Only |
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