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TCP-1α monoclonal antibody (91a)

ADI-CTA-191-D 50 µg 243.00 USD
ADI-CTA-191-F 200 µg 501.00 USD
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Replaces Prod. #: ALX-804-107

Product Details

Alternative Name:CCT, p63, Tric, T-complex 1
Immunogen:Recombinant mouse TCP-1α (C-terminal fragment).
UniProt ID:P11983
GenBank ID:D90344
Source:Purified from mouse ascites.
Species reactivity:Human, Mouse, Rat
Bovine, C. elegans, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Plant, Rabbit, Yeast
Applications:Flow Cytometry, ICC, IHC, IP, WB
Recommended Dilutions/Conditions:Flow Cytometry (1:100)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~60kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:TCP-1 alpha is a 60 kDa subunit of a cytosolic hetero-oligomer chaperone that is known to be involved in the folding of Actin and Tubulin. This protein is a member of the Chaperonin family, which includes E. coli GroEL, the mitochondrial Heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein, and the archaebacterial protein TF55. These Chaperonins assist the folding of proteins upon ATP hydrolysis.
Technical Info/Product Notes:Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000120438).
Regulatory Status:RUO - Research Use Only
TCP-1α monoclonal antibody (91a) Flow Cytometry
Flow cytometric analysis human colon cancer Coca-2 cells were analyzed by FLOW cytometry using isotype control antibody (left) or TCP-1alpha antibody, clone #91a (right).
TCP-1α monoclonal antibody (91a) Western blot
Western blot analysis of CTA-191: Lane 1: MW marker, Lane 2: HeLa Cell Lysate, Heat shocked, Lane 3: PC-12 Cell Lysate Heat Shocked, Lane 4: 3T3 Cell Lysate, Heat Shocked, Lane 5, RK-13 Cell Lysate, Heat Shocked.
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TCP-1α monoclonal antibody (91a) Flow Cytometry TCP-1α monoclonal antibody (91a) Western blot

Product Literature References

A 102 kDa subunit of a Golgi-associated particle has homology to beta subunits of trimeric G proteins: K.R. Willison, et al. ; EMBO J. 12, 2847 (1993), Application(s): IP using human samples, Abstract;
T-complex polypeptide-1 is a subunit of a heteromeric particle in the eukaryotic cytosol: K. Willison, et al. ; Nature 358, 249 (1992), Application(s): IP (M), ICC (MO) using mouse & monkey samples, Abstract;

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