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ARF1 monoclonal antibody (ARFS 3F1)

ADI-CSA-450-D 50 µg 213.00 USD
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Product Specification

Alternative Name:ADP ribosylation factor 1
Clone:ARFS 3F1
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human ARF1.
UniProt ID:P84077
Source:Purified from mouse ascites.
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Monkey, Porcine, Rabbit, Sheep
Recommended Dilutions/Conditions:Western Blot (ECL, 1:250)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~22kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:ADP-ribosylation factors (ARF) are a ubiquitous family of GTP-binding proteins involved in membrane dynamics and vesicular trafficking.  The ARF family also includes the structurally related ARF-like proteins.  ARF family members possess a highly conserved structure and function in eukaryotes.  ARFs function as essential proteins in Saccharomyces cerevisiae (ARF-1 and ARF-2) and in Drosophila melanogaster (ARF-like).  Many studies associate ARF proteins with exocytic and endocytic transport pathways, and the activation of phospholipase D activity.  ARF-1, an abundantly expressed Ras-like N-myristoylated GTP-binding protein, co-purifies with tightly bound GDP.  ARF possesses an extra β-strand (β2E) and N-terminal helix, two structural elements missing in Ras.  Like Ras, ARF-1 switches between the GTP-bound “on” state where it transfers signals to its effectors in the signaling pathway, and the GDP-bound “off” state.
Regulatory Status:RUO - Research Use Only
ARF1 monoclonal antibody (ARFS 3F1) Western blot
Western Blot Analysis of ARF1: Lane 1: MWM, Lane 2: Rat Brain, Lane 3: Mouse Brain, Lane 4: MDCK.
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ARF1 monoclonal antibody (ARFS 3F1) Western blot

Product Literature References

The mechanism and function of mitogen-activated protein kinase activation by ARF1: F. Zhou, et al.; Cell. Signal. 27, 2035 (2015), Application(s): Western Blot, Abstract;

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