Product Details
Alternative Name: | ACTA, ACTSA |
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Host: | Rabbit |
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Immunogen: | Bovine skeletal muscle Actin. |
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UniProt ID: | P68133 |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human, Mouse, Rat Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
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Applications: | WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~42kDa by Western blot. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Actin is one of the major proteins (~42 kDa) of the cytoskeleton. It regulates contractile potential in muscle tissues and helps to control the shape as well as the motility of non-muscle cells. Actin is expressed in all eukaryotic cells which makes it a useful loading control in Western blotting experiments. In higher eukaryotes, there are six isoforms of actin: Three alpha-actins (α-skeletal, α-cardiac, α-smooth muscle), one β-actin (β-non-muscle) and two γ-actins (γ-smooth muscle and γ-non-muscle). Actin isoforms show >90% overall sequence homology. |
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Regulatory Status: | RUO - Research Use Only |
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Western Blot Analysis of Actin pAb: Lane 1: MWM, Lane 2: Mouse Heart Tissue Extract, Lane 3: Chicken Heart Tissue Extract.
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Product Literature References
Neuroprotective and neurotoxic outcomes of androgens and estrogens in an oxidative stress environment: P. Duong, et al.; Biol. Sex Differ.
11, 12 (2020),
Abstract;
Full Text
Effect of HSP25 loss on muscle contractile function and running wheel activity in young and old mice: K.A. Huey, et al.; Front. Physiol.
4, 398 (2013),
Application(s): WB using muscle samples,
Abstract;
Full Text
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell
134, 769 (2008),
Application(s): WB using human cell lysates,
Abstract;
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