Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

EGF receptor monoclonal antibody (6F1)

ADI-CSA-330-E 100 µg 362.00 USD
Do you need bulk/larger quantities?

Product Details

Alternative Name:EGFR, epidermal growth factor receptor
Immunogen:Recombinant human EGF receptor (C-terminal fragment).
UniProt ID:P00533
Species reactivity:Human
Applications:Flow Cytometry, ICC, IP, WB
Recommended Dilutions/Conditions:Flow cytometry: 1:100 (final concentration)
Immunocytochemistry: 1:100
Immunoprecipitation: 2-5 µL/250 µL of cell extract from 2.5 x 106 cells
Western Blot: 1:1,000-1:2,000 for chemiluminescence detection system

Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~170kDa by Western blot.
Purity Detail:Ammonium sulfate precipitation purified.
Formulation:Liquid. In PBS, pH 7.2 containing 50% glycerol.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:The epidermal growth factor receptor (EGFR) is a 170-kDa membrane bound receptor consisting of an extracellular ligand-binding domain, a single hydrophobic transmembrane region, and a tyrosine kinase domain-containing cytoplasmic region. Ligand binding induces receptor homo- or heterodimerization, triggering autophosphorylation of cytoplasmic tyrosine residues that provide docking sites for Src homology 2 (SH2) domain-containing signaling molecules. Src-mediated phosphorylation of EGFR at Tyr845 may contribute to EGFR hyperactivity in cancer, while autophosphorylation of EGFR at Tyr1068 and/or Tyr1173 in response to ligand binding is known to recruit cofactors (e.g. SHP1, RasGAP, SOS1, Grb2) that activate pathways associated with cell growth, apoptosis, adhesion, receptor endocytosis, and protein degradation.
Regulatory Status:RUO - Research Use Only
EGF receptor monoclonal antibody (6F1) Western blot
Western blot analysis of human A431 cell lysate probed with EGFR mAb (6F1).
EGF receptor monoclonal antibody (6F1) Immunoprecipitation
Immunoprecipitation analysis of human EGF -R from A431 cells with 2µg (1) and 10μg (2) of EGFR mAb (6F1), and mouse IgG (3).
Please mouse over
EGF receptor monoclonal antibody (6F1) Western blot EGF receptor monoclonal antibody (6F1) Immunoprecipitation

Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation: S. Yoon, et al.; Immunity 47, 51 (2017), Abstract;
Differential regulation of epidermal growth factor receptor by hydrogen peroxide and flagellin in cultured lung alveolar epithelial cells: H. Nishi, et al.; Eur. J. Pharmacol. 748, 133 (2015), Application(s): Western blot, Abstract;
Phosphorylation of epidermal growth factor receptor at serine 1047 by MAP kinase-activated protein kinase-2 in cultured lung epithelial cells treated with flagellin: N. Noguchi, et al.; Arch. Biochem. Biophys. 529, 75 (2013), Application(s): Western blot, Abstract;
Selective cleavage of ErbB4 by G-protein-coupled gonadotropin-releasing hormone receptor in cultured hypothalamic neurons: S. Higa-Nakamine, et al.; J. Cell. Physiol. 227, 2492 (2012), Abstract;

Related Products

Goat anti-mouse IgG, polyclonal antibody (AP conjugate) 

ELISA, WB | Print as PDF
ADI-SAB-101-J 1 ml 170.00 USD
Do you need bulk/larger quantities?

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
EGFR Monoclonal antibody
By catalog section:


Technical Service
Customer Service