Product Specification
| Alternative Name: | CASPER, CLARP, I-FLICE, Usurpin |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of human c-FLIP6. The sequence conserved among all c-FLIP variants. |
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| UniProt ID: | O15519 |
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| GenBank ID: | U97074, U97075 |
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| Species reactivity: | Human, Mouse, Rat
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| Applications: | ICC, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (5µg/ml)
Western Blot (0.5-1.0µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~28-58kDa by Western blot. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The cellular FLICE-like inhibitory protein, cFLIP, is a cellular homologue of the viral protein, vFLIP. cFLIP contains two death effector domains (DEDs) and is expressed as either a short or long splice variant called cFLIPs and cFLIPL, respectively. cFLIP acts as a caspase-8 dominant negative regulator, blocking apoptosis induced by the oligimerization of the adapter protein, FADD. cFLIP interacts with FADD and caspase-8 through their respective DEDs. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunocytochemistry analysis of FLIP in HeLa cells with FLIP antibody at 5µg/ml.
Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2µg/ml.
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Product Literature References
RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation: G. Liccardi, et al.; Mol. Cell
73, 413 (2019),
Abstract;
Full Text
Codium fragile F2 sensitize colorectal cancer cells to TRAIL-induced apoptosis via c-FLIP ubiquitination: S.H. Park, et al.; Biochem. Biophys. Res. Commun.
508, 1 (2018),
Abstract;
Involvement of Up-Regulation of DR5 Expression and Down-Regulation of c-FLIP in Niclosamide-Mediated TRAIL Sensitization in Human Renal Carcinoma Caki Cells: J.M. Yun, et al.; Molecules
23, E2264 (2018),
Abstract;
Full Text
Ophiopogonin B sensitizes TRAIL-induced apoptosis through activation of autophagy flux and downregulates cellular FLICE-like inhibitory protein: U.M. Nazim, et al.; Oncotarget
9, 4161 (2017),
Abstract;
Full Text
The coffee diterpene kahweol enhances sensitivity to sorafenib in human renal carcinoma Caki cells through down-regulation of Mcl-1 and c-FLIP expression: K.J. Min, et al.; Oncotarget.
8, 83195 (2017),
Abstract;
Full Text
5 Fluorouracil Preferentially Sensitizes Mutant KRAS Non-Small Cell Lung Carcinoma Cells to TRAIL-Induced Apoptosis: H. Wang, et al.; Mol. Oncol.
9, 1815 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Activation of surrogate death receptor signaling triggers peroxynitrite-dependent execution of cisplatin-resistant cancer cells: S. Seah, et al.; Cell Death Dis.
6, e1926 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL: J. Sun, et al.; Int. J. Clin. Exp. Pathol.
8, 6519 (2015),
Application(s): Western blot ,
Abstract;
Full Text
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