Product Details
Alternative Name: | APIP |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of human Apaf-1. |
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UniProt ID: | O14727 |
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GenBank ID: | AF013263 |
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Species reactivity: | Human, Mouse, Rat Chicken
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Applications: | IHC, WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (1µg/ml) Western Blot (1-2µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~120kDa by Western blot. |
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Purity Detail: | Peptide affinity purified. |
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Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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Shipping: | Blue Ice Not Frozen |
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Long Term Storage: | -20°C |
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Scientific Background: | Apaf-1 (Apoptosis protease-activating factor 1), a mammalian protein homologous to C. elegans CED-4, is an essential adaptor molecule and a key linker of the mitochondrial apoptotic pathway. Apaf-1 binds to Cytochrome c (Apaf-2) leading to the formation of a large multimeric complex. This complex recruits (through CARD) Procaspase-9 and once activated, caspase-9 (Apaf-3) dissociates. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 can also associate with caspase-4, caspase-8 and Diva. Apaf-1 is ubiquitously expressed in human tissues. |
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Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of Apaf1 in human heart tissue with Apaf1 antibody at 1µg/ml.
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at 1µg/ml in the (A) absence and (B) presence of blocking peptide.
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Product Literature References
Y Nerve growth factor blocks thapsigargin-induced apoptosis at the level of the mitochondrion via regulation of Bim: A. Samali, et al. ; J. Cell. Mol. Med.
12, 2482 (2008),
Application(s): WB using rat cell lysates,
Abstract;
Death in the balance: alternative participation of the caspase-2 and -9 pathways in neuronal death induced by nerve growth factor deprivation: M.L. Shelanski, et al. ; J. Neurosci.
21, 5007 (2001),
Application(s): WB using mouse samples,
Abstract;
The adapter protein apoptotic protease-activating factor-1 (Apaf-1) is proteolytically processed during apoptosis: S. Wesselborg, et al. ; J. Biol. Chem.
276, 29772 (2001),
Application(s): WB using human samples,
Abstract;
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