Replaces Prod. #: ALX-804-245
Product Details
| Alternative Name: | Cyt c |
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| Clone: | 7H8.2C12 |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Immunogen: | Horse Cytochrome c . |
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| UniProt ID: | P00004 |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Guinea pig, Hamster, Monkey, Rabbit, Sheep
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| Applications: | IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~14kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Cytochrome c is an electron transport protein from mitochondria. A primary means of caspase activation by p53 is through induction of cytochrome c release from mitochondria. Cleavage of Bid, a Bcl 2 family member, by caspase-8 produces a truncated tBid molecule which triggers mitochondria release of cytochrome c which binds to the adaptor protein Apaf-1 and procaspase-9 to form a cytosolic apoptosome complex. This event results in activation of procaspase-9 and its subsequent cleavage and activation of caspase-3 leading to the degradation of a series of cellular substrates and subsequently cell death. |
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| Regulatory Status: | RUO - Research Use Only |
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IHC Analysis: Human uterus tissue stained with Cytochrome c, mAb (7H8.2C12) at 5µg/ml.
IHC Analysis: Human spleen tissue stained with Cytochrome c, mAb (7H8.2C12) at 5µg/ml.
IHC Analysis: Human brain, cortex tissue stained with Cytochrome c, mAb (7H8.2C12) at 5µg/ml.
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