Product Details
| Clone: | 5B4 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human caspase-9 (N-terminal fragment). |
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| UniProt ID: | P55211 |
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| GenBank ID: | AAC50640 |
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| Species reactivity: | Human, Mouse, Rat
Drosophila
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~46-50kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Apoptosis is a major form of cell death characterized by several morphological features that include chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. These morphological changes occur via signalling pathways that lead to the recruitment and activation of caspases, a family of cysteine-containing, aspartate-specific proteases. Caspases exist as inactive proenzymes in cells, and are activated through their processing into two subunits in response to apoptotic stimulation. Activated caspases cleave a variety of important cellular proteins, other caspases, and Bcl-2 family members, leading to a commitment to cell death. Caspase-9, also known as ICE-LAP61, Mch-62 or Apaf-33 is a 45 kDa protein that is involved in one of the relatively well characterized caspase cascades. It is triggered by Cytochrome C release from the mitochondria, which promotes the activation of caspase-9 by forming a complex with Apaf-1 in the presence of dATP. Once activated, caspase-9 initiates a caspase cascade involving caspase-3, -6, and -7. The active form of caspase-9 is composed of a 32-35 kDa subunit and 10 kDa subunit. Caspase-9 is expressed in a variety of tissues. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Caspase-9 fragments expression in apoptosis induced Jurkat cells by Fas mAb, probed with Caspase-9 mAb (5B4).
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Product Literature References
Lipid emulsion attenuates propranolol-induced early apoptosis in rat cardiomyoblasts: S.H. Ok, et al.; Hum. Exp. Toxicol.
41, 9603271221110852 (2022),
Abstract;
AZD1208, a Pan-Pim Kinase Inhibitor, Has Anti-Growth Effect on 93T449 Human Liposarcoma Cells via Control of the Expression and Phosphorylation of Pim-3, mTOR, 4EBP-1, S6, STAT-3 and AMPK: A.K. Yadav, et al.; Int. J. Mol. Sci.
20, 363 (2019),
Abstract;
Full Text
Glycolysis promotes caspase-3 activation in lipid rafts in T cells: M.A. Secinaro, et al.; Cell Death Dis.
9, 62 (2018),
Abstract;
Full Text
LY3009120, a pan-Raf kinase inhibitor, inhibits adipogenesis of 3T3-L1 cells by controlling the expression and phosphorylation of C/EBP-α, PPAR-γ, STAT‑3, FAS, ACC, perilipin A, and AMPK: S.M. Yang, et al.; Int. J. Mol. Med.
42, 3477 (2018),
Abstract;
Meridianin C inhibits the growth of YD-10B human tongue cancer cells through macropinocytosis and the down-regulation of Dickkopf-related protein-3: N.S. Park, et al.; J. Cell. Mol. Med.
22, 5833 (2018),
Abstract;
Full Text
Novel carbazole sulfonamide microtubule-destabilizing agents exert potent antitumor activity against esophageal squamous cell carcinoma: F. Niu, et al.; Cancer Lett.
420, 60 (2018),
Abstract;
Gardenin B-induced cell death in human leukemia cells involves multiple caspases but is independent of the generation of reactive oxygen species: J. Cabrera, et al.; Chem. Biol. Interact.
256, 220 (2016),
Application(s): Western blot,
Abstract;
Rapid cold-hardening protects Drosophila melanogaster from cold-induced apoptosis: R.E. Lee, et al. ; Apoptosis
12, 1183 (2007),
Application(s): WB using drosophila cell lysates,
Abstract;
Role of Smac in human leukaemic cell apoptosis and proliferation: A.C. Newland, et al. ; Oncogene
22, 1589 (2003),
Application(s): WB using human samples,
Abstract;
Bax and Bak independently promote cytochrome C release from mitochondria: E. White, et al. ; J. Biol. Chem.
277, 14127 (2002),
Application(s): WB using mouse samples,
Abstract;
Dysfunctional apoptosome activation in ovarian cancer: implications for chemoresistance: G. Nunez, et al. ; Cancer Res.
62, 924 (2002),
Application(s): WB using human samples,
Abstract;
Insights into regulation and function of the major stress-induced hsp70 molecular chaperone in vivo: analysis of mice with targeted gene disruption of the hsp70.1 or hsp70.3 gene: D. Moskophidis, et al. ; Mol. Cell. Biol.
21, 8575 (2001),
Application(s): WB using mouse samples,
Abstract;
