- Cost effective- coat your own plates and stretch your research budget
- Quantitative - obtain fully quantitative statistically significant results
- Sensitive - detect as little as 49 pg/mL HO-1 compared to microgram levels in Western blot
- Reliable - obtain accurate results with thoroughly tested reagents in multiple complex sample matrices
The IMMUNOSET® HO-1 (human), EIA development set contains sufficient reagents for the development of 5 x 96 well EIA plates which provides analysis of ~200 samples in duplicate. Save time, money, and sample by developing your own EIA kits. Simply coat your plates and go! This kit provides superior accuracy with fully quantitative results compared to Western blot analysis and has low reactivity with related molecules.
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Product Details
| Alternative Name: | Heme oxygenase 1 |
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| Sensitivity: | 49 pg/ml (range 0.195 - 12.5 ng/ml) |
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| Assay Time: | Plate Coating - Overnight + 1 hour; Assay - 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human HO-1 in cell lysates and tissue of human origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Crossreactivity: | Specific for human HO-1; does not react with human HO-2 or HO-3. |
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| Contents: | Capture antibody, Standard, Detection antibody, SA-HRP |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Heme oxygenase (Hsp32) is the rate-limiting enzyme that breaks down heme to iron, carbon monoxide, and biliverdin, which is then metabolized to bilirubin by biliverdin reductase. In mammalians, heme oxygenase exists as two primary isoforms, the inducible isoform HO-1, and the constitutively expressed HO-2, both catalyzing the same reaction. HO-1 is expressed in erythrocyte and hemoglobin metabolizing tissues of the spleen, liver, and bone marrow, with localization to membranes of the ER, mitochondria, and caveolae2. HO-1 expression is induced in response to an array of oxidative stress-inducing factors, including heat shock, heme accumulation, hypoxia, UV radiation, nitric oxide, cytokines, and heavy metals. |
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| UniProt ID: | P09601 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome: Y. Hara, et al.; Can. Respir. J.
2018, 9627420 (2018),
Application(s): Human serum,
Abstract;
Full Text
Oxidative stress and the altered reaction to it in Fabry disease: A possible target for cardiovascular-renal remodeling?: V. Ravarotto, et al.; PLoS One
13, e0204618 (2018),
Application(s): Cell lysates of human PBMCs,
Abstract;
Full Text
Vascular and metabolic effects of the haem oxygenase-1 inducer haem arginate in subjects with the metabolic syndrome: A translational cross-over study: D. Dekker, et al.; Diab. Vasc. Dis. Res.
13, 41 (2016),
Application(s): HO-1 levels in human plasma,
Abstract;
HO-1 inhibits IL-13-induced goblet cell hyperplasia associated with CLCA1 suppression in normal human bronchial epithelial cells: K. Mishina, et al.; Int. Immunopharmacol.
29, 448 (2015),
Application(s): ELISA for HO-1 protein in cell lysates,
Abstract;
Renal cortical hemopexin accumulation in response to acute kidney injury: R.A. Zager, et al. ; Am. J. Physiol. Renal. Physiol.
303, F1460 (2012),
Application(s): HO-1 levels in HEK293 and HK-2 cell lysates,
Abstract;
Full Text
