- Flexible - suitable for use in a variety of species and sample matrices
- Higher throughput - assay ~200 samples in a fraction of the time compared to Western blot
- Accurate - fully quantitative results surpass semi-quantitative Western blot analysis
The IMMUNOSET® Grp94 ELISA development set contains sufficient reagents for the development of 5 x 96 well ELISA plates which provides analysis of ~200 samples in duplicate. Save time, money, and sample by developing your own ELISA kits. Simply coat your plates and go! This kit provides superior accuracy with fully quantitative results compared to Western blot analysis and has low reactivity with related molecules.
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Product Specification
| Alternative Name: | Endoplasmin, Tra1, Hsp90B1, Gp96 |
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| Sensitivity: | 1.29 ng/ml (range 6.25 - 200 ng/ml) |
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| Assay Time: | Plate Coating - Overnight + 1 hour; Assay - 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of Grp94 in cell lysates and tissue of human, mouse, rat, and dog origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human, Mouse, Rat
Dog
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| Crossreactivity: | This assay detects Grp94 in cell lysates and microsomes. Cross reactivity with human Calreticulin, PDI, HSP90α, HSP90β, HSP70, hamster Grp78 and bovine Hsc70 is less than 0.035%. |
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| Contents: | Capture antibody, Standard, Detection antibody, SA-HRP |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Grp94 (Glucose-regulated protein 94) is an abundant resident endoplasmic reticulum (ER) lumenal stress protein, which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. Grp94 expression is upregulated by stress conditions such as glucose starvation and heat shock, which promote protein misfolding or unfolding. In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. |
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| UniProt ID: | P14625 (human) |
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| Regulatory Status: | RUO - Research Use Only |
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