- Accurate - fully quantitative results surpass semi-quantitative Western blot analysis
- Reliable - thoroughly validated in complex mouse and rat sample matrices
- Cost effective - assay ~200 samples in duplicate for the cost of one ready-to-use ELISA
The IMMUNOSET® HSP25 (rodent), ELISA development set contains sufficient reagents for the development of 5 x 96 well ELISA plates which provides analysis of ~200 samples in duplicate. Save time, money, and sample by developing your own ELISA kits. Simply coat your plates and go! This kit provides superior accuracy with fully quantitative results compared to Western blot analysis and has low reactivity with related molecules.
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Product Details
| Sensitivity: | 0.38 ng/ml (range 0.78 - 25 ng/ml) |
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| Assay Time: | Plate Coating - Overnight + 1 hour; Assay - 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of HSP25 in cell lysates of mouse and rat origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Mouse, Rat
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| Crossreactivity: | Specific for HSP25. Does not cross-react with HSP27, Cpn10, HO-1, HSP40, or αB-crystallin |
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| Contents: | Capture antibody, Standard, Detection antibody, SA-HRP |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Hsp25 is a member of the family of small heat shock proteins (sHsps), an evolutionarily conserved family of stress proteins which exhibit moleclular weights between 14-45 kDa. The sHsps are related to the alpha-crystallins, and generally exist as large oligomeric complexes that serve to stabilize and assist in refolding of denatured proteins. Hsp25 is highly homologous to the human Hsp27, and its expression is seen in many species, including mouse, rat, bovine, canine, and hamster cells. |
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| UniProt ID: | P14602 (mouse), P42930 (rat) |
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| Regulatory Status: | RUO - Research Use Only |
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